Protein Info for PFLU_RS12010 in Pseudomonas fluorescens SBW25

Annotation: amino acid metabolite efflux pump

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 33 to 51 (19 residues), see Phobius details amino acids 58 to 78 (21 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 115 to 133 (19 residues), see Phobius details amino acids 139 to 159 (21 residues), see Phobius details amino acids 171 to 190 (20 residues), see Phobius details amino acids 205 to 225 (21 residues), see Phobius details amino acids 235 to 256 (22 residues), see Phobius details amino acids 263 to 281 (19 residues), see Phobius details PF00892: EamA" amino acids 7 to 131 (125 residues), 64.1 bits, see alignment E=7.5e-22 amino acids 141 to 278 (138 residues), 52.6 bits, see alignment E=2.8e-18

Best Hits

Swiss-Prot: 37% identical to EAMA_ECOLI: Probable amino-acid metabolite efflux pump (eamA) from Escherichia coli (strain K12)

KEGG orthology group: K03298, drug/metabolite transporter, DME family (inferred from 100% identity to pfs:PFLU2451)

MetaCyc: 37% identical to cysteine/O-acetylserine exporter EamA (Escherichia coli K-12 substr. MG1655)
RXN0-1923; RXN0-1924

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8M2 at UniProt or InterPro

Protein Sequence (300 amino acids)

>PFLU_RS12010 amino acid metabolite efflux pump (Pseudomonas fluorescens SBW25)
MLKKHLSLAVLVTLVWGVNFPITKLGLRAIDPFVLTGIRFALAALPLVFFIKRPAVKFSY
VAAYGFIFGLGMWGVINYGIQVGVSPGIASLIIQLSVFFTMGWGFVLFKEKIRGAQMIGA
VLALIGLAGIISTQQGNHAVLGVMLIVLSAVAWSVGNVIIKKSGVKEIFSFMVWASLIPP
IPLFLTAWLMHGSAAFEGLQASLDLTAILSILFQVYLATHFAYWGWNSLLKLYPVSTVAP
LSLLIPVFGITSSMLMLDERISTPNLISIGIIIAGLAVGLYRRPANLAPTSQGAVMQAEK