Protein Info for PFLU_RS11900 in Pseudomonas fluorescens SBW25

Annotation: type II secretion system protein GspF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 403 transmembrane" amino acids 168 to 191 (24 residues), see Phobius details amino acids 221 to 240 (20 residues), see Phobius details amino acids 261 to 284 (24 residues), see Phobius details amino acids 374 to 396 (23 residues), see Phobius details TIGR02120: type II secretion system protein F" amino acids 4 to 402 (399 residues), 535.2 bits, see alignment E=6e-165 PF00482: T2SSF" amino acids 70 to 192 (123 residues), 112.2 bits, see alignment E=8e-37 amino acids 272 to 394 (123 residues), 104.7 bits, see alignment E=1.7e-34

Best Hits

Swiss-Prot: 46% identical to GSPF_PSEAE: Type II secretion system protein F (xcpS) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K02455, general secretion pathway protein F (inferred from 100% identity to pfs:PFLU2425)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8K0 at UniProt or InterPro

Protein Sequence (403 amino acids)

>PFLU_RS11900 type II secretion system protein GspF (Pseudomonas fluorescens SBW25)
MNRYRYEAADASGKVEAGHVEADSQSAAFASLRSRGLTALLVQIEGGQPAAAGSSLFTAK
LSDNDLAWATRQLASLLGASLPLEAALSATVEQAEKKHIAQTLAGVRADVRGGMRLAEAL
AARPRDFPSIYRALIAAGEESGDLAQVMERLADYIEERNNLRGKILTAFIYPGVVGLVSI
GIVIFLLSYVVPQVVSAFSQARQDLPGLTLAMLNASDFIRAWGWLCFAGIVGGFWSWRLY
LRNPVARLNWHSRVLRLPLIGRFVLGLNTARFASTLAILGGAGVPLLRALEAARQTLSND
RLSQCVNDATAKVREGVNLAPALAVEKVFPPVLIHLIASGEKTGSLPPMLERAAQTLSRD
IERRAMGMTALLEPLMIVVMGAVVLVIVMAVLLPIIEINQLVT