Protein Info for PFLU_RS11875 in Pseudomonas fluorescens SBW25

Annotation: general secretion pathway protein GspK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 276 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF21687: T2SSK_1st" amino acids 88 to 171 (84 residues), 34.9 bits, see alignment E=3.4e-12 PF03934: T2SSK" amino acids 176 to 234 (59 residues), 50.3 bits, see alignment E=3.9e-17

Best Hits

KEGG orthology group: K02460, general secretion pathway protein K (inferred from 100% identity to pfs:PFLU2420)

Predicted SEED Role

"General secretion pathway protein K"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K9B5 at UniProt or InterPro

Protein Sequence (276 amino acids)

>PFLU_RS11875 general secretion pathway protein GspK (Pseudomonas fluorescens SBW25)
MAIISALLIAAVVAVLAGAMLTRQTVFTRSLEAEQLRIQGQWLLQGGLERSRQMLWDARQ
KEVLTRLDQSWARAQGGAFEGRIEDEQGKFNLRNLVNRQQVDSEQLQSFERLCQLIGVDP
AVSRRVSQRVIASYLPPVKYPMLRSLDDLSGLEGLDPLVLQRMQAYISVLPGPTWVNGNT
ASAEVLSAVVPQLSLSQAHGLVAERDSGQWFINRGDFVNRLHLPQVVMDSVQVGITSEWF
RLQGQARREQRRVTIDALLHRPEDRQPRVIWSRVGV