Protein Info for PFLU_RS11655 in Pseudomonas fluorescens SBW25

Annotation: L-arabinose ABC transporter ATP-binding protein AraG

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 508 PF00005: ABC_tran" amino acids 29 to 178 (150 residues), 104.9 bits, see alignment E=5.7e-34 amino acids 280 to 433 (154 residues), 71.7 bits, see alignment E=1e-23

Best Hits

Swiss-Prot: 86% identical to ARAG_PSEPF: Arabinose import ATP-binding protein AraG (araG) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K10539, L-arabinose transport system ATP-binding protein [EC: 3.6.3.17] (inferred from 100% identity to pfs:PFLU2373)

MetaCyc: 62% identical to arabinose ABC transporter ATP binding subunit (Escherichia coli K-12 substr. MG1655)
ABC-2-RXN [EC: 7.5.2.12, 7.5.2.13]

Predicted SEED Role

"L-arabinose transport ATP-binding protein AraG (TC 3.A.1.2.2)" in subsystem L-Arabinose utilization (TC 3.A.1.2.2)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.17

Use Curated BLAST to search for 3.6.3.17 or 7.5.2.12 or 7.5.2.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K971 at UniProt or InterPro

Protein Sequence (508 amino acids)

>PFLU_RS11655 L-arabinose ABC transporter ATP-binding protein AraG (Pseudomonas fluorescens SBW25)
MHAQLQTHNAAAQLSFNGIGKTFPGVKALDGISFTAYPGQVHALMGENGAGKSTLLKILG
GAYIPSSGTLQIGEQTMAFKNAADSIASGVAVIHQELHLVPEMTVAENLFLGHLPSRFGV
VNRSLLRQQALACLKGLADEIDPQEKLGRLSLGQRQLVEIAKALSRGAHVIAFDEPTSSL
SAREIDRLMAIITRLRDEGKVVLYVSHRMEEVFRICNAVTVFKDGRYVRTFDDMSALTHD
QLVTCMVGRDIQDIYDYRPREQGEVALKVDGLLGPGLREPISFNVRKGEILGLFGLVGAG
RTELFRLLSGLERASAGQLELCGQALQLRSPRDAIAAGVLLCPEDRKKEGIIPLSSVAEN
INISARGAHSAFGWLLRDGWEKRNADQQIQAMKVKTPNAAQKIMYLSGGNQQKAILGRWL
SMPMKVLLLDEPTRGIDIGAKAEIYQIIHNLAAQGIAVIVVSSDLMEVMGISDRILVLCE
GAMRGEQTREHATESNLLQLALPRSLAN