Protein Info for PFLU_RS11250 in Pseudomonas fluorescens SBW25
Annotation: AMP-binding protein
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01895, acetyl-CoA synthetase [EC: 6.2.1.1] (inferred from 100% identity to pfs:PFLU2290)Predicted SEED Role
"Acetyl-coenzyme A synthetase (EC 6.2.1.1)" in subsystem Ketoisovalerate oxidoreductase or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 6.2.1.1)
MetaCyc Pathways
- ethanol degradation II (3/3 steps found)
- ethanol degradation IV (3/3 steps found)
- superpathway of acetate utilization and formation (3/3 steps found)
- acetate and ATP formation from acetyl-CoA III (1/1 steps found)
- acetate conversion to acetyl-CoA (1/1 steps found)
- L-isoleucine biosynthesis V (2/3 steps found)
- ethanol degradation III (2/3 steps found)
- chitin deacetylation (2/4 steps found)
- reductive glycine pathway of autotrophic CO2 fixation (5/9 steps found)
- cis-geranyl-CoA degradation (4/9 steps found)
- adlupulone and adhumulone biosynthesis (1/6 steps found)
- colupulone and cohumulone biosynthesis (1/6 steps found)
- lupulone and humulone biosynthesis (1/6 steps found)
- superpathway of bitter acids biosynthesis (3/18 steps found)
KEGG Metabolic Maps
- Glycolysis / Gluconeogenesis
- Propanoate metabolism
- Pyruvate metabolism
- Reductive carboxylate cycle (CO2 fixation)
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.1
Use Curated BLAST to search for 6.2.1.1
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K562 at UniProt or InterPro
Protein Sequence (560 amino acids)
>PFLU_RS11250 AMP-binding protein (Pseudomonas fluorescens SBW25) MTQPFLAARDFLLAHRTDYATAVRDFRWPQLGEFNWALDYFDAMAEGNPANALWIVEEDG SEQRYSFQQLAARANQVANHLRALGVRRGDRVLLMLGNDVALWETMLAAFKLGAVVIPAT ALLNADDLRDRIERGQVRHLVVGEAHVSKFAGLADGCSRICVGSAPAGWVAHSAAFAYPE HFEAEGRTLATDPMLLYFTSGTTSKPKMVLHSHQSYPVGHLSTMYWIGLQPGDLHLNISS PGWAKHAWSCLFAPWNAGACIFIHNVARFSAPTLLSTLERYGVTSLCAPPTVWRMLIQED LASYKPRLSLRELVGAGEPLNPEIIEQIQQAWGLPLRDGFGQSETTALVGNTPGQLLKPG SMGRPLPGYQVALLDPDGNPGTEGEVALPLDVRPLGLMLCYEDSPEKTAEVMRDGYYRTG DTAQIDADGYITFVGRADDVFKASDYRISPFELESALIEHPAVMEVAVVPSPDPLRLAVP KAFLILAHDAPGSAELARHILAFAREHLAPYKRVRRIEFVSELPKTISGKIRRVELRQME VLRRQSNTRGEQEHFEEDFG