Protein Info for PFLU_RS11010 in Pseudomonas fluorescens SBW25

Annotation: cystathionine beta-lyase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 918 PF00581: Rhodanese" amino acids 6 to 101 (96 residues), 43 bits, see alignment E=5.1e-15 amino acids 130 to 223 (94 residues), 48.1 bits, see alignment E=1.4e-16 amino acids 280 to 355 (76 residues), 37.2 bits, see alignment E=3.4e-13 amino acids 375 to 463 (89 residues), 28.7 bits, see alignment E=1.5e-10 PF01053: Cys_Met_Meta_PP" amino acids 537 to 914 (378 residues), 334.4 bits, see alignment E=6.7e-104 TIGR01324: cystathionine beta-lyase" amino acids 537 to 917 (381 residues), 410.3 bits, see alignment E=3.1e-127

Best Hits

KEGG orthology group: K01760, cystathionine beta-lyase [EC: 4.4.1.8] (inferred from 100% identity to pfs:PFLU2239)

Predicted SEED Role

"Rhodanese-related sulfurtransferase / Cystathionine beta-lyase (EC 4.4.1.8)" in subsystem Methionine Biosynthesis (EC 4.4.1.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.4.1.8

Use Curated BLAST to search for 4.4.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K945 at UniProt or InterPro

Protein Sequence (918 amino acids)

>PFLU_RS11010 cystathionine beta-lyase (Pseudomonas fluorescens SBW25)
MSQTVTPGQLQQWLFDGQEIALFDVREHGQYGEAHLFFGVNLPYSRLELEVWRLAPNPKV
RLVIYDQNGGDIAARSAERLQALGYEHVHVLEGGADGWQAAGLQLFAGVHVPSKAFGELV
EETSHTPHVTAQQLAEWQARGEPLVVLDGRPFDEYRKMTIPGSVCCPNGELGYRLQDLVP
DDHTPVVVNCAGRTRSIIGAQTLINLGVKNPVYALENGTQGWYLEDFQLEHGSTRRYADD
VSSKALAQQRLAAQKLAEQAGAKTVLAAQVEQWAADVTRSLFLCDVRTAEEFAAGSLPGA
QHTPGGQLIQSTDLYIGVRQARLVLIDSDGVRAPIVASWLRQLGHEAYVLAGGVSSGLAL
PAYQAPAYQALPVISAKSLSDALKDDTLVVIDLRPSMSYRKAHIAGSRWSIRSLLASDVA
GDQRPLVLLADDPRLAAFAAQVLPDTQRAHARLLEGGLDAWRAAGFAVQEDANTPPDHQC
IDFLFFTHDRHSGNKDAARQYLAWEIGLLAQMSANEIASLKPLRVQPQATLDARVRTRLV
HAARTEKGQGGRGVNVPVSRLSTVIFDNLTQMRDARARRDTERVLSYGARGNPTAFALED
MVTELEGGYRTRLFGTGLAAVAQTFLAYLRPGDHVLITDAVYAPVRRLARDLLEPFGIQV
NYFAPDGSDLQAQLQANTKMVYTEVPGSLLYELGDLPAIAALCKPRDILLAVDNTWGSGY
LYRPLALGADISIMALTKYLCGHSDVVMGSVCTRQDVWQPLASMSDTVGIAVSPDDAYLV
LRGARTLAARMEVHERQALDIAHWLQAQPQVKRVFHPALPDHPNHALWRRDFAGSNGLLS
FELRNDEVTGVERFIDALQVFGLGASWGGYESLVTVADPSDRNSVSDRQLNPVLRLHIGL
EDVGALIEDLQRGFAALG