Protein Info for PFLU_RS10915 in Pseudomonas fluorescens SBW25

Annotation: ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 527 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF00496: SBP_bac_5" amino acids 75 to 416 (342 residues), 296.9 bits, see alignment E=1.2e-92

Best Hits

KEGG orthology group: K02035, peptide/nickel transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU2220)

Predicted SEED Role

"Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) or Sex pheromones in Enterococcus faecalis and other Firmicutes (TC 3.A.1.5.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8D1 at UniProt or InterPro

Protein Sequence (527 amino acids)

>PFLU_RS10915 ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25)
MRSFSFRRALTSVALCTTLLAGQAQAQPQEGGVLNLVAQPEPPSLMHGVVSHVSTQYVSG
KVLQGLLTFDTHLAPKPVLAKAWTISPDGLTYTFDLQDCVHWHDGPAFTADDVVFSFQTF
YPEVDKRLGGIITEYVESITAKGTLQVVFHLKKPFAPLLSALGSGLRPVVPKHLYENTDF
RNNPYNLKPVGTGPFVFVEWKRGAYIKLAKNPNYWKKGLPHLDSIIFHVIPDASSRAAAF
ERNDVQVLRSGDADYSDLKRLTALPDVQSSEKGWELYSGLAFLQINTRKPPLNNLKVRQA
ILSALNRQFIVDNIFFGSGKVAQGPFVSSSPYHDPQLPQYAYDVKKAKALIAESGVDAGA
VRIRLLNGEKGGAWERLAEYTKQSLQPLGFKVQVVTSDAATWFQRVSDWDFDLTYNFIFQ
IGDPYLTSAYLFRSDYILKTSPFANVSGYNSAEADALWAKVADTPEGPERKQLYSQLENL
LNTDLPIAPIFEMRYPTLFHKQVKNLLQTATSLNEDYESVYLEALTP