Protein Info for PFLU_RS10725 in Pseudomonas fluorescens SBW25

Annotation: transporter substrate-binding domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1206 signal peptide" amino acids 1 to 25 (25 residues), see Phobius details transmembrane" amino acids 537 to 559 (23 residues), see Phobius details PF00497: SBP_bac_3" amino acids 65 to 275 (211 residues), 88.8 bits, see alignment E=1.8e-28 amino acids 304 to 520 (217 residues), 108.5 bits, see alignment E=1.6e-34 PF00989: PAS" amino acids 579 to 688 (110 residues), 42.4 bits, see alignment E=3e-14 TIGR00229: PAS domain S-box protein" amino acids 583 to 697 (115 residues), 37.1 bits, see alignment E=1.5e-13 PF08448: PAS_4" amino acids 585 to 696 (112 residues), 46 bits, see alignment E=2.6e-15 PF00512: HisKA" amino acids 714 to 780 (67 residues), 57.3 bits, see alignment 5.9e-19 PF02518: HATPase_c" amino acids 828 to 942 (115 residues), 87.4 bits, see alignment E=4.2e-28 PF00072: Response_reg" amino acids 966 to 1079 (114 residues), 69.8 bits, see alignment E=9.9e-23 PF01627: Hpt" amino acids 1124 to 1194 (71 residues), 34.9 bits, see alignment 6.8e-12

Best Hits

KEGG orthology group: K07679, two-component system, NarL family, sensor histidine kinase EvgS [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU2181)

Predicted SEED Role

"Hybrid sensory histidine kinase in two-component regulatory system with EvgA" in subsystem Orphan regulatory proteins

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7S3 at UniProt or InterPro

Protein Sequence (1206 amino acids)

>PFLU_RS10725 transporter substrate-binding domain-containing protein (Pseudomonas fluorescens SBW25)
MTTFRRLKLTAILLLGLLPYSAMALDEPHTLRLLGHSSLEHPSVELDESDWRWLRERRTL
VMGVSAPDYAPFDLSNDNDFEGITADYTWLISLILNVPIEVRRYDTRDEVIEALKLGNVD
LVGSANGYEAADKALVLSRSYANDQPVLVTRAGDSHSLSVDLAGKRVAMLYHYMQPDAVQ
AFYPQAQLLLYGSTHQAIGSVAFGQADVYLGDAISTRYLINKNHLNNVRMADFSALEVNP
FGFAFTGDNIRLLKIVNAALAVIPAGQQMEILRRWSAGGSGFLEADRLRLSDSEQRWLEK
HPRVKVAALDKFMPLSFFNEQGQFIGLSDEVLSRISLRTGLKFEVVQGSSLPRQVDEVSA
GQVEMMAVITPSVERSEKVRFTRPYLSNPYVLVARASDEHLLTLEDMPGKRLAFIGGNSL
AVQVARDYPGIVFVAVENPEQAMELVAKGGVDATVVSLISARYMIARHYRDRLRITSTVG
TEPARIALGVNRSQLELYSILEKALLSITPEEMDELTNHWRSEIIIEDSYWISHRNVIIQ
GFGLAAVLLLVTLGWVLYLRNLIHKRAQAERALSDQMRFMSVLIDGTPHPIYVRDRQGRL
MACNNAYLDVFGFKLEDVIGKTVVETDTGNPPQAQSFHADYLSLMERGEPQIHDRVLKVP
GGGVLTIYQWMLPYRDSNGNVVGMIAGWVDVSERQRLLGQLQEAKEEADAANRAKTTFLA
TMSHEIRTPMNAVIGMIELAQKNAEQGRVDQDALEVASMASRSMLELIGDILDIARIETG
HLSLTLAPANLHEMLASVARVFEGLARDKGLLLHVELDPMIDRSVLIDPMRFKQVVSNLL
GNAIKFTATGQVRLGAQCTPALASDPLNLRLYVEDTGIGISAEDQQRLFNPFIQGSNNDQ
SARSGSGLGLVISRNLCEMMGGQLHLSSVLGKGTRVDVTLALELATAAPLSSSAPPVPPA
HALSLLVVDDYPANRLLLTRQLSFLGHRIVTAEDGAEGFALWQAGHFDGVITDSNMPVMD
GYTLARDIRAQERRLGLVPCLLLGFTANAQPEETERCRQAGMDGCLFKPTGLDDLRVALA
SRTASAAVEDAEPTFDLSALVMLTGGEGAVLDELLLPLLNSLAEDRLLLPALQQHADFAK
LHDLAHRIKGGARMVRAQALVAGCETLEEACQRRDRDALSAAVEAVGMAVDGLHQRLSLH
LHSKHA