Protein Info for PFLU_RS10690 in Pseudomonas fluorescens SBW25

Annotation: FAD-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 649 PF01266: DAO" amino acids 61 to 97 (37 residues), 29 bits, see alignment 1.2e-10 PF13450: NAD_binding_8" amino acids 64 to 101 (38 residues), 27.8 bits, see alignment 3.6e-10 PF01593: Amino_oxidase" amino acids 69 to 125 (57 residues), 24.7 bits, see alignment 2.1e-09 amino acids 355 to 606 (252 residues), 71.1 bits, see alignment E=1.8e-23

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2174)

Predicted SEED Role

"Tryptophan 2-monooxygenase (EC 1.13.12.3)" in subsystem Aromatic amino acid degradation (EC 1.13.12.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.13.12.3

Use Curated BLAST to search for 1.13.12.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7A6 at UniProt or InterPro

Protein Sequence (649 amino acids)

>PFLU_RS10690 FAD-dependent oxidoreductase (Pseudomonas fluorescens SBW25)
MTIGSNFNYSADIAQRHHRKKRSSKNKWRATYPNPPDLCFDYRALVEQVNGIALATETQH
KICIIGAGITGLTTARELYRCGFTNLTLLEKSTRIGGRHLTALGHNNTNTVGRPPFEMGA
MRMPFFNHSSEPPKEGRSLMAYYANAFELRHSDFPNPGSPAVRSTGIYLREGSIDNSTEP
TMLIWKNADGKTPPPGQTLGNVFAKWKVFAERMTLIIAEHYGAEHWEDMWASIVRTYEGI
SFRDLVKMPSIDNWNQSNPGNFGGMGMTAQESAVFYAIGIGDGSWGAFYDVCALYPLRTA
IFGFSSHLQLIHGRVNADGTLMASPYLDAPETLDSKGIAFNKPNYIGLGSLAESLLFIKP
GETQDSLYEHLIKSQSGLLTSSAVTKLKKLNNGQIRVYYDWSIAPKGTSSSAFIDCDSVV
MTLPSWLIETQVSIEGFSQEMLPFEITEAYKSAHWETSCKVYAPLKKSFLSHNKNIPQTL
VTDSLVHDVYTYRYNNTNTYDCILLSYTWEDDATKLSVFSDEELVDKCIEDLDRILLKST
NIKEKISPYIGRKQAVVQRWMNDKNSLGCAKLYRAGTYYNAMKLLQYNRNFSHKSGLYLC
GESFSVDAGWTEPCFRGAVDTVINICNKTRATFNGGFTMHDYPTYKAEP