Protein Info for PFLU_RS10450 in Pseudomonas fluorescens SBW25

Annotation: TRAP transporter large permease subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 20 to 41 (22 residues), see Phobius details amino acids 52 to 79 (28 residues), see Phobius details amino acids 99 to 115 (17 residues), see Phobius details amino acids 118 to 137 (20 residues), see Phobius details amino acids 146 to 171 (26 residues), see Phobius details amino acids 193 to 214 (22 residues), see Phobius details amino acids 235 to 254 (20 residues), see Phobius details amino acids 260 to 279 (20 residues), see Phobius details amino acids 292 to 310 (19 residues), see Phobius details amino acids 331 to 350 (20 residues), see Phobius details amino acids 358 to 387 (30 residues), see Phobius details amino acids 421 to 438 (18 residues), see Phobius details PF13726: Na_H_antiport_2" amino acids 3 to 86 (84 residues), 100.5 bits, see alignment E=7.4e-33 PF03553: Na_H_antiporter" amino acids 150 to 434 (285 residues), 274.5 bits, see alignment E=1.7e-85 PF06808: DctM" amino acids 184 to 390 (207 residues), 38.1 bits, see alignment E=1.3e-13

Best Hits

Swiss-Prot: 58% identical to Y2115_VIBPA: Uncharacterized membrane protein VP2115 (VP2115) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K07084, (no description) (inferred from 100% identity to pfs:PFLU2124)

Predicted SEED Role

"Histidine permease YuiF"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K692 at UniProt or InterPro

Protein Sequence (440 amino acids)

>PFLU_RS10450 TRAP transporter large permease subunit (Pseudomonas fluorescens SBW25)
MINAVIAAVGTMLVLSLSRVHVVIAIIVGALVGGLTGGLGIDATLKAFNGGLGGGATVAL
SYALLGAFAVAIAKSGLAHALADKALLLVDRQEATGGSHVKWLLIGLLWVVAIASQNILP
IHIAFIPLLVPPLLYVLTKLQLDRRLIACVMTFGLITPYMFLPVGFGNIFLNQILLANVA
KSGVDISQVNVTHAMSLPALGMVVGLLVAVFVSYRKKRVYDLEKIERVEQVAVQYNPLTL
LVAGVAIASAFIIQLWLDSMIIGALAGFLIFSVSGIVRWRDTDDLFTEGMKMMAMIGFIM
IASSGFAEVLKATGDVRSLVETSAAFIGHSRGVGALLMLLVGLLVTMGIGSSFSTVPILA
AIFVPLCVQLGFSPVAIVCIVGTAGALGDAGSPASDSTLGPTSGLNIDGQHHHIWDTVVP
TFLHYNLPLLAFGWLAAMTL