Protein Info for PFLU_RS10125 in Pseudomonas fluorescens SBW25
Annotation: aminotransferase class V-fold PLP-dependent enzyme
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 59% identical to ISCS_AZOVI: Cysteine desulfurase IscS (iscS) from Azotobacter vinelandii
KEGG orthology group: K04487, cysteine desulfurase [EC: 2.8.1.7] (inferred from 100% identity to pfs:PFLU2060)MetaCyc: 52% identical to cysteine desulfurase IscS (Escherichia coli K-12 substr. MG1655)
Cysteine desulfurase. [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]; 2.8.1.7 [EC: 2.8.1.7]
Predicted SEED Role
"Cysteine desulfurase (EC 2.8.1.7)" in subsystem Alanine biosynthesis (EC 2.8.1.7)
MetaCyc Pathways
- superpathway of L-alanine biosynthesis (4/4 steps found)
- superpathway of thiamine diphosphate biosynthesis II (9/11 steps found)
- superpathway of thiamine diphosphate biosynthesis I (8/10 steps found)
- L-alanine biosynthesis III (1/1 steps found)
- L-cysteine degradation IV (1/1 steps found)
- thiazole component of thiamine diphosphate biosynthesis II (5/7 steps found)
- molybdopterin biosynthesis (4/6 steps found)
- thiazole component of thiamine diphosphate biosynthesis I (4/6 steps found)
- cytidylyl molybdenum cofactor sulfurylation (1/2 steps found)
- bis(guanylyl molybdopterin) cofactor sulfurylation (1/3 steps found)
- tRNA-uridine 2-thiolation (mammalian mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (yeast mitochondria) (1/4 steps found)
- tRNA-uridine 2-thiolation (thermophilic bacteria) (1/5 steps found)
- [2Fe-2S] iron-sulfur cluster biosynthesis (4/10 steps found)
- tRNA-uridine 2-thiolation and selenation (bacteria) (4/11 steps found)
- tRNA-uridine 2-thiolation (cytoplasmic) (1/8 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.8.1.7
Use Curated BLAST to search for 2.8.1.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K526 at UniProt or InterPro
Protein Sequence (386 amino acids)
>PFLU_RS10125 aminotransferase class V-fold PLP-dependent enzyme (Pseudomonas fluorescens SBW25) MNKRPLYFDYAATTPVDERVIQVMVECLGFNANFGNPASSSHAFGQAARQTVEQARRQVA ELVGAQPEQIVWTSGATESNNLAIKGVAQARGVAGGHIITSQIEHKATLDTARQLQEAGV AVTYLVPDADGLISVEAVSEALREDTFLVSLMLVNNELGTLNDIPAIGARVREHGALLHV DAAQGAGKVAIDLAQWPVDLMSFSAHKLYGPKGIGALYVGPRAQQKVLAQIHGGGHEGGL RSGTLATHQIAGMGTAFALAAASFAEEKAVIVALRQRLLDQLESIAGVRLNGSATQRIPH TLSLTFSEGEFNAAALSAGIAFSATSACNSASNTPSHVLLALGHDARSAGRTIRLSLGRF TTEQDIDQAVQLIKATLASAPAFWAV