Protein Info for PFLU_RS10100 in Pseudomonas fluorescens SBW25

Annotation: sigma-54-dependent Fis family transcriptional regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 367 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details PF00158: Sigma54_activat" amino acids 24 to 187 (164 residues), 241.8 bits, see alignment E=5e-76 PF14532: Sigma54_activ_2" amino acids 27 to 192 (166 residues), 76.6 bits, see alignment E=3.6e-25 PF07728: AAA_5" amino acids 45 to 164 (120 residues), 35.6 bits, see alignment E=1.3e-12

Best Hits

Swiss-Prot: 90% identical to SFNR_PSEPF: Sigma54-dependent transcriptional regulator SfnR (sfnR) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU2055)

Predicted SEED Role

"Alkanesulfonates-binding protein" in subsystem Alkanesulfonate assimilation or Alkanesulfonates Utilization or Putative sulfate assimilation cluster

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K930 at UniProt or InterPro

Protein Sequence (367 amino acids)

>PFLU_RS10100 sigma-54-dependent Fis family transcriptional regulator (Pseudomonas fluorescens SBW25)
MQLLTLPPSPALATSIRATAQVFEDPKSQALLDHIQQVAPSEASVLIIGETGTGKELVAR
HIHNLSARRNRPFVAVNCGAFSESLVEAELFGHEKGAFTGALSAKAGWFEEADGGTLFLD
EIGDLPMAIQVKLLRVLQEREVVRLGSRKSIAIDVRVLAATNVQLEKAINAGHFREDLYY
RLDVVSLELSPLRERPGDILPLTRHFIDAYSQRLGYGPITISREAEHKLKSYSWPGNIRE
LENVIHHTLLICRNGVIERDDLRLSNMRIERQDDSQHGTDNSAEALLARAFQKLFEEQAG
ALHEKVEDALLRAAYRFSHYNQVHTANLLGLSRNVTRTRLIKIGELAVNKRRPGENVQGE
RMLHLSI