Protein Info for PFLU_RS09610 in Pseudomonas fluorescens SBW25
Annotation: histidinol dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 65% identical to HISX2_RHILO: Histidinol dehydrogenase 2 (hisD2) from Mesorhizobium japonicum (strain LMG 29417 / CECT 9101 / MAFF 303099)
KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 100% identity to pfs:PFLU1949)MetaCyc: 50% identical to sulfopropanediol 3-dehydrogenase monomer (Cupriavidus pinatubonensis JMP134)
RXN-11727 [EC: 1.1.1.308]
Predicted SEED Role
"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
- L-histidine biosynthesis (10/10 steps found)
- 3-sulfopropanediol degradation I (1/3 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.1.1.23
Use Curated BLAST to search for 1.1.1.23 or 1.1.1.308
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K6Q4 at UniProt or InterPro
Protein Sequence (427 amino acids)
>PFLU_RS09610 histidinol dehydrogenase (Pseudomonas fluorescens SBW25) MIRQLKQGRPAEAKAELNAQVRTTVEEILAAIEHRGEAALREYSSQFDNWAPDDFRLTSE QIQACIDSLSPEALDDIRFAQAQIRRFAQIQLHSLRDVEVETMPGVVLGHRNIPVNSVGC YIPGGKYPLLASAHMSVLTAKVAGVKRVIACAPPFGGKPSNEIVAAMALAGADEIYVMGG VQGVAAMALGTEKIAPVDMIVGPGNAYVAEAKRQLFGRVGIDLFAGPTETMVICDDSVDA ELVAVDLLGQAEHGPTSPAYCLTTSTRIAEELPAAIEQVLSRLDTAPVASVAWRDYGEII LCDSDEEMLAESERLCSEHVQVMTRDPDWFLERMTCYGGLFLGHRTNVSYGDKVIGTNHT LPTQGAGRYTGGLWVGKFIKTHTYQRILTDEASVAMGEVCSRLCALEHFSGHKEQADIRV RRLKKTV