Protein Info for PFLU_RS09320 in Pseudomonas fluorescens SBW25

Annotation: IS21 family transposase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 516 PF00665: rve" amino acids 136 to 248 (113 residues), 36.4 bits, see alignment E=2.6e-13

Best Hits

Swiss-Prot: 37% identical to Y4UI_SINFN: Putative transposase y4uI (NGR_a01310) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1887)

Predicted SEED Role

"Mobile element protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K883 at UniProt or InterPro

Protein Sequence (516 amino acids)

>PFLU_RS09320 IS21 family transposase (Pseudomonas fluorescens SBW25)
MRLPISTQREVLRLTSDPKLSNRAIGLLAKVSHNTVRTIRDQLAQCGQSWEELKHLDDKA
LAERLHTQPKVSSQRKAYPSWPAIHEQLKLPDITLELLWQEFRTSEPEGVSYAQFTRIYR
EWVNRQKLSMRQTHLPGDKCFVDFCGRTMPVTNPDTGAISQAQVFVATLGASGYIFATAV
RSQTIPDWLRCNILALEFFDGVPRFVVPDNLKSAVTKNTRDVVTLNRAYAEFSEHYGFQI
YPARPRKPQDKSLGEIAVQLVQRFVLARLRASTFFSLEELNEKISYWVDELNSRITRTYP
KSRTSRFLELDYPALKPLPEHKYSYSKWVYQVRVGSDYHVQFEEHSYSVPYHFANLLVDL
RVRDDWLEIIHQRRLIGSHKLNRQRGTSTLKEHLAPNHRYFQDSQPEALIVWAETIGPET
LRFVKDNLNLRKDFSTGLKVVVGLKRDVRKGAITPSRLESACGYANSLNILSSERLRSIL
RNESDLRPNYRRSAPLVEHANIRGAEYYASQGDEPL