Protein Info for PFLU_RS08945 in Pseudomonas fluorescens SBW25

Annotation: succinate dehydrogenase flavoprotein subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 590 TIGR01816: succinate dehydrogenase, flavoprotein subunit" amino acids 8 to 590 (583 residues), 991.8 bits, see alignment E=8.3e-303 TIGR01812: succinate dehydrogenase or fumarate reductase, flavoprotein subunit" amino acids 10 to 590 (581 residues), 836.4 bits, see alignment E=1.1e-255 PF00890: FAD_binding_2" amino acids 10 to 407 (398 residues), 394.1 bits, see alignment E=1.4e-121 PF02910: Succ_DH_flav_C" amino acids 462 to 590 (129 residues), 151.5 bits, see alignment E=1.9e-48

Best Hits

Swiss-Prot: 69% identical to SDHA_ECOL6: Succinate dehydrogenase flavoprotein subunit (sdhA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K00239, succinate dehydrogenase flavoprotein subunit [EC: 1.3.99.1] (inferred from 100% identity to pfs:PFLU1818)

MetaCyc: 92% identical to succinate dehydrogenase flavoprotein subunit (Pseudomonas putida KT2440)
Succinate dehydrogenase (ubiquinone). [EC: 1.3.5.1]

Predicted SEED Role

"Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1)" in subsystem Serine-glyoxylate cycle or Succinate dehydrogenase or TCA Cycle (EC 1.3.99.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.1

Use Curated BLAST to search for 1.3.5.1 or 1.3.99.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6M5 at UniProt or InterPro

Protein Sequence (590 amino acids)

>PFLU_RS08945 succinate dehydrogenase flavoprotein subunit (Pseudomonas fluorescens SBW25)
MANIPTISFDAIIIGGGGAGMRAALQLAQGGHKTAVITKVFPTRSHTVSAQGGITCAIAS
ADPNDDWRWHMYDTVKGSDYIGDQDAIEYMCQEGPAAVFELDHMGLPFSRTEQGRIYQRP
FGGQSKDYGKGGQAARTCAASDRTGHALLHTLYQGNLKAGTTFLNEYYAVDLVKNAQGEF
VGVIAICIETGETTYIRAKATVLATGGAGRIYASTTNALINTGDGVGMALRAGVPVQDIE
MWQFHPTGIAGAGVLVTEGCRGEGGYLINKHGERFMERYAPNAKDLAGRDVVARSMVKEI
IAGNGCGPNGDHVLLKLDHLGEEVLHSRLPGICELSKTFAHVDPVVAPVPVVPTCHYMMG
GVPTNIHGQAITQNADGVDEIIHGLFAVGEVACVSVHGANRLGGNSLLDLVVFGRAAGLH
LEKALTDGIEYDDATDANIEAALARLNALNERTDGEDVATLRRELQSCMQNYFGVFRTGE
YMQKGIAQLADLRTRIANVKINDKSQAFNTARIEALELQNLLEVAEATAIAAEVRKESRG
AHAREDFEDRDDENWLCHTLYFPGDKRVTKRAVNFSPKTVPTFEPKIRTY