Protein Info for PFLU_RS08835 in Pseudomonas fluorescens SBW25

Annotation: sulfate ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 334 signal peptide" amino acids 1 to 23 (23 residues), see Phobius details TIGR00971: sulfate ABC transporter, sulfate-binding protein" amino acids 19 to 330 (312 residues), 431.9 bits, see alignment E=7.1e-134 PF13531: SBP_bac_11" amino acids 31 to 279 (249 residues), 123.3 bits, see alignment E=2.1e-39 PF13416: SBP_bac_8" amino acids 88 to 282 (195 residues), 30.1 bits, see alignment E=6.5e-11

Best Hits

Swiss-Prot: 54% identical to SUBI_ECOLI: Sulfate-binding protein (sbp) from Escherichia coli (strain K12)

KEGG orthology group: K02048, sulfate transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU1796)

MetaCyc: 54% identical to sulfate/thiosulfate ABC transporter periplasmic binding protein Sbp (Escherichia coli K-12 substr. MG1655)
ABC-19-RXN [EC: 7.3.2.5]; ABC-7-RXN [EC: 7.3.2.5, 7.3.2.3]; 7.3.2.3 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-478 [EC: 7.3.2.5, 7.3.2.3]; TRANS-RXN0-479 [EC: 7.3.2.5, 7.3.2.3]

Predicted SEED Role

"Sulfate and thiosulfate binding protein CysP" in subsystem Cysteine Biosynthesis

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.3.2.3 or 7.3.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5N1 at UniProt or InterPro

Protein Sequence (334 amino acids)

>PFLU_RS08835 sulfate ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25)
MKKLVSASLLAAGLALAGAVHAAPVTLLNVSYDVMRDFYKDYNAAFQKHWEAEHPNDKLT
LQMSFGGSSKQARSVIDGLPADVITMNMATDINALVDNGKLVPDNWVTRLPNNSAPFTSA
TVFIVRKGNPKALKDWPDLLKDGVQVIVPNPKTSGNGRYTYLSAWGYVLKNGGDENKAKA
FVGKLFKQAPVLDTGGRAATTTFMTNQIGDVLVTFENEAEMIAREFGRDQFEVIYPSVSA
EAEPPVSVVDKVVEKKGTRVAAEDYLKYLWSPEGQEIAANNYLRPRDPKVLAKYTDRFPK
VDFLSVEKTFGDWRTVQKTHFNDGGVFDQIYSGQ