Protein Info for PFLU_RS08765 in Pseudomonas fluorescens SBW25

Annotation: phosphonate C-P lyase system protein PhnH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 196 PF05845: PhnH" amino acids 8 to 191 (184 residues), 197.1 bits, see alignment E=1.2e-62 TIGR03292: phosphonate C-P lyase system protein PhnH" amino acids 10 to 190 (181 residues), 172.8 bits, see alignment E=3.3e-55

Best Hits

KEGG orthology group: K06165, PhnH protein (inferred from 100% identity to pfs:PFLU1782)

Predicted SEED Role

"PhnH protein" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5L7 at UniProt or InterPro

Protein Sequence (196 amino acids)

>PFLU_RS08765 phosphonate C-P lyase system protein PhnH (Pseudomonas fluorescens SBW25)
MSAPLLQPAFVDPVLDAQRGFRAALKALAEPGLIQHLPCAPRLDGLAPATYALCLALLDT
DTPLWLAPSFDTPLIRANLAFHCGCPLASKREDAVFALLGEQDLLDLSGFDHGNDRYPDQ
SCTLLVQLTDLEGGRGLLWRGPGIKAQRQVHLPVPQGFWHERQRREAFPRGLDVVFAAGH
HLIGLPRSSRIAQEHA