Protein Info for PFLU_RS08755 in Pseudomonas fluorescens SBW25

Annotation: phosphonate metabolism transcriptional regulator PhnF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 225 TIGR02325: phosphonate metabolism transcriptional regulator PhnF" amino acids 1 to 223 (223 residues), 168.1 bits, see alignment E=1.2e-53 PF00392: GntR" amino acids 2 to 62 (61 residues), 52.9 bits, see alignment E=2.3e-18 PF07702: UTRA" amino acids 84 to 220 (137 residues), 95.4 bits, see alignment E=2.8e-31

Best Hits

KEGG orthology group: K02043, GntR family transcriptional regulator, phosphonate transport system regulatory protein (inferred from 100% identity to pfs:PFLU1780)

Predicted SEED Role

"Transcriptional regulator PhnF" in subsystem Alkylphosphonate utilization

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5L5 at UniProt or InterPro

Protein Sequence (225 amino acids)

>PFLU_RS08755 phosphonate metabolism transcriptional regulator PhnF (Pseudomonas fluorescens SBW25)
MYRELADILRRELSSYQAGDFLPGEVHMAERFGVNRHTLRRAIDELVFEGSLLRRQGKGT
QVLDRPLIYPMGAETSYSQSLSAQGVGVEALLLKRRYCYASREEAQHLGIAEMAPMIELQ
TLRKLDHQPVSLIRHRYCASRAPLLADYTGGSLRHYLRERDLPLTRTHSLIGARLPNRDE
AALLMMPRHLPALTVFTLSRDRDGHPVELAQSTSRSDRFQYQVMT