Protein Info for PFLU_RS08700 in Pseudomonas fluorescens SBW25

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 483 signal peptide" amino acids 1 to 18 (18 residues), see Phobius details transmembrane" amino acids 33 to 50 (18 residues), see Phobius details amino acids 62 to 81 (20 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 120 to 140 (21 residues), see Phobius details amino acids 149 to 171 (23 residues), see Phobius details amino acids 183 to 205 (23 residues), see Phobius details amino acids 211 to 228 (18 residues), see Phobius details amino acids 249 to 271 (23 residues), see Phobius details amino acids 283 to 306 (24 residues), see Phobius details amino acids 314 to 333 (20 residues), see Phobius details amino acids 339 to 362 (24 residues), see Phobius details amino acids 381 to 401 (21 residues), see Phobius details amino acids 453 to 471 (19 residues), see Phobius details PF07690: MFS_1" amino acids 5 to 390 (386 residues), 150.9 bits, see alignment E=4.5e-48 PF00083: Sugar_tr" amino acids 12 to 174 (163 residues), 28 bits, see alignment E=1e-10

Best Hits

Swiss-Prot: 49% identical to LFRA_MYCS2: Multidrug efflux pump LfrA (lfrA) from Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)

KEGG orthology group: K08167, MFS transporter, DHA2 family, methyl viologen resistance protein SmvA (inferred from 100% identity to pfs:PFLU1769)

Predicted SEED Role

"Unknown, probable transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4Z7 at UniProt or InterPro

Protein Sequence (483 amino acids)

>PFLU_RS08700 MFS transporter (Pseudomonas fluorescens SBW25)
MLPVLLVTVDNTVLGFALPKISEALHPSASQQLWMIDAYSLVLAGLLISMGSLGDRKGHR
KLLLIGSLGFTVVSVMTAYSSTAPQLIVGRACMGIFGAMLMPSTLALIRSVFEDREERRL
AVAIWATTLTVGSALGPLVGGVLLEFFSWGSIFLLAVPVLVPLLVLGPWLLPESEPDASG
PMDIISTVQSMIALSALVYSIKHIASDGPDALALAVFGVAIVIGWQFVRRQLRLPIPLMD
LSLFRNKTFSGSVTINLMSLAFLIGFVFFSTQFLQIALRMSPLSASIALIPGQLMAIAVG
ILIVPVAQRVSVNVLIPILLAFTAGAFLLVAGLGSSLTVLVAAFALLNIGVGAIATVSND
VILSAVPSTKAGAASAISETAYEVGVVLGTTLLGGLVTAYYRGTLQLPDFLTPVQMHLAS
ETLAGAHAISATLAHDQASEVMDNAIRAFEGGTHLMAWATFSLAVLAIVVARRTLKAPQT
APA