Protein Info for PFLU_RS08105 in Pseudomonas fluorescens SBW25
Annotation: bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00800, 3-phosphoshikimate 1-carboxyvinyltransferase [EC: 2.5.1.19] (inferred from 100% identity to pfs:PFLU1647)Predicted SEED Role
"Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19)" in subsystem Chorismate Synthesis or Common Pathway For Synthesis of Aromatic Compounds (DAHP synthase to chorismate) or Phenylalanine and Tyrosine Branches from Chorismate (EC 1.3.1.12, EC 1.3.1.43, EC 2.5.1.19)
MetaCyc Pathways
- superpathway of aromatic amino acid biosynthesis (18/18 steps found)
- superpathway of L-tryptophan biosynthesis (13/13 steps found)
- superpathway of L-phenylalanine biosynthesis (10/10 steps found)
- superpathway of L-tyrosine biosynthesis (10/10 steps found)
- chorismate biosynthesis I (7/7 steps found)
- chorismate biosynthesis from 3-dehydroquinate (5/5 steps found)
- L-tyrosine biosynthesis I (3/3 steps found)
- L-tyrosine biosynthesis III (3/4 steps found)
- superpathway of chorismate metabolism (42/59 steps found)
- chorismate biosynthesis II (archaea) (8/12 steps found)
- 4-hydroxy-3-prenylbenzoate biosynthesis (1/4 steps found)
- superpathway of novobiocin biosynthesis (4/19 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Novobiocin biosynthesis
- Phenylalanine, tyrosine and tryptophan biosynthesis
Isozymes
Compare fitness of predicted isozymes for: 1.3.1.12, 2.5.1.19
Use Curated BLAST to search for 1.3.1.12 or 1.3.1.43 or 2.5.1.19
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K6J8 at UniProt or InterPro
Protein Sequence (748 amino acids)
>PFLU_RS08105 bifunctional prephenate dehydrogenase/3-phosphoshikimate 1-carboxyvinyltransferase (Pseudomonas fluorescens SBW25) MVDVTALQPAVPMIGRLVVVGLGLIGGSFAKGLRESGLCGEVVGVDLDPQSRTLAVELGV VDRCEADLALACQGADVIQLAVPILAMEKLLAVLAGMDLGQAILTDVGSAKGNVVRAAQQ AFGGMPARFVPGHPIAGSEQSGVEASNAQLFRRHKVILTPLAQTDPAALAVVDRLWRELG ADVEHMQVERHDEVLAATSHLPHLLAFGLVDSLAKRNENLEIFRYAAGGFRDFTRIAGSD PVMWHDIFLANREAVLRTLDTFRSDLDALRDAVDAGDGHQLLGVFTRARVAREHFSKILA RRAYMETAVNTDDLTFLANPGGRLSGRIRVPGDKSISHRSIMLGSLAEGVTEVEGFLEGE DALATLQAFRDMGVVIEGPHHGRVTIHGVGLHGLKPAPGPIYLGNSGTSMRLLSGLLAAQ RFDSVLTGDASLSKRPMNRVAKPLRDMGAVIETGPEGRPPLTIRGGQALKGLTYAMPMAS AQVKSCLLLAGLYAEGKTAVTEPAPTRDHTERMLRGFGYPVAVEGATASLESGHALIATH IEVPGDISSSAFFLVAASIAEGSELLLEHVGVNPTRTGVIEILRLMGADITLENQREVGG EPVADLRVRAASLKGIEIPEALVPLAIDEFPVLFVAAACAEGRTVLRGAQELRVKESDRI QVMADGLLALGVKCEPTPDGIIIDGGLMGGGDVHAHGDHRIAMAFSVASLRAAAPIRIHD CANVATSFPNFLTLCAQVGIRVAQEAQL