Protein Info for PFLU_RS08030 in Pseudomonas fluorescens SBW25

Annotation: proline iminopeptidase-family hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 295 TIGR01250: proline-specific peptidase" amino acids 24 to 290 (267 residues), 271.6 bits, see alignment E=4.8e-85 PF00561: Abhydrolase_1" amino acids 31 to 279 (249 residues), 88.3 bits, see alignment E=9.9e-29 PF12697: Abhydrolase_6" amino acids 33 to 283 (251 residues), 66.9 bits, see alignment E=6.5e-22 PF12146: Hydrolase_4" amino acids 50 to 140 (91 residues), 37.5 bits, see alignment E=2.5e-13

Best Hits

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 100% identity to pfs:PFLU1631)

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K632 at UniProt or InterPro

Protein Sequence (295 amino acids)

>PFLU_RS08030 proline iminopeptidase-family hydrolase (Pseudomonas fluorescens SBW25)
MWREIAPDQQYNVQVDGHNLVVYSFGEGDEVLLCLNGGPGLPCDYLRDAHGWLKEHNLRV
VAFDQLGTGASARPTDVSLWEIGRYVEEVETVRQALGLGRVHLLGHSWGGWLGIEYAIHY
PDALKSLILENTVGDIPHLSQELERLRGALGSETVAMMQRHEAMGTLDHPQYQAAITLLN
YRHVCRLDEWPEPVKRSLGDWNMGPYETMQGPNEFLYIGNLKDWNRIPEMAAFTMPILIT
TGQHDELTPACALRMKMAAKHAQLHVFPNSSHMPFYEEPQAYFPVLLDFLARHRG