Protein Info for PFLU_RS08020 in Pseudomonas fluorescens SBW25

Annotation: L-amino acid amidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 310 TIGR01250: proline-specific peptidase" amino acids 9 to 296 (288 residues), 249.6 bits, see alignment E=2.4e-78 PF00561: Abhydrolase_1" amino acids 33 to 284 (252 residues), 82.7 bits, see alignment E=5.1e-27 PF12146: Hydrolase_4" amino acids 34 to 284 (251 residues), 42.3 bits, see alignment E=8.4e-15 PF12697: Abhydrolase_6" amino acids 34 to 287 (254 residues), 52.5 bits, see alignment E=1.7e-17

Best Hits

Swiss-Prot: 100% identical to LAAA_PSEFS: L-amino acid amidase (laaA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01259, proline iminopeptidase [EC: 3.4.11.5] (inferred from 100% identity to pfs:PFLU1629)

MetaCyc: 97% identical to L-proline amide hydrolase (Pseudomonas azotoformans)
RXN-10757 [EC: 3.5.1.101]; 3.5.1.101 [EC: 3.5.1.101]

Predicted SEED Role

"Proline iminopeptidase (EC 3.4.11.5)" in subsystem Proline, 4-hydroxyproline uptake and utilization (EC 3.4.11.5)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.5

Use Curated BLAST to search for 3.4.11.5 or 3.5.1.101

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K630 at UniProt or InterPro

Protein Sequence (310 amino acids)

>PFLU_RS08020 L-amino acid amidase (Pseudomonas fluorescens SBW25)
MEFIEKIREGYAAFGAYQTWYRVTGDLSSGRTPLVVIHGGPGCTHDYVDAFKDVAASGHA
VIHYDQLGNGRSTHLPDKDPSFWTVGLFLEELNNLLDHLQISDNYAILGQSWGGMLGSEH
AILQPKGLRAFIPANSPTCMRTWVSEANRLRKLLPEGVHETLLKHEAAGTYQDPEYLAAS
RVFYDHHVCRVNPWPEEVARTFAQVDADPTVYHAMSGPTEFHVIGSLKDWKSIGRLSAIN
VPTLVISGRHDEATPLVVKPFLDEIADVRWALFEDSSHMPHVEERQACMGTVVKFLDEVC
SVPHNVLKAG