Protein Info for PFLU_RS07890 in Pseudomonas fluorescens SBW25

Annotation: response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 transmembrane" amino acids 23 to 43 (21 residues), see Phobius details amino acids 297 to 317 (21 residues), see Phobius details PF00512: HisKA" amino acids 459 to 524 (66 residues), 66.8 bits, see alignment 2.1e-22 PF02518: HATPase_c" amino acids 571 to 685 (115 residues), 98.6 bits, see alignment E=4.4e-32 PF00072: Response_reg" amino acids 824 to 931 (108 residues), 88.6 bits, see alignment E=4.9e-29

Best Hits

KEGG orthology group: K07677, two-component system, NarL family, capsular synthesis sensor histidine kinase RcsC [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU1603)

Predicted SEED Role

"Signal transduction histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4W7 at UniProt or InterPro

Protein Sequence (940 amino acids)

>PFLU_RS07890 response regulator (Pseudomonas fluorescens SBW25)
MKLKHFLQPLDATFSTPKAARKLLRLFALALVLGVYAGAYSYLRAAFSEEISLRRSYMNE
AISDAQSFFVSRQTLLKSLGLSTVRHVTPPVGNLNNVPAEEVHITLGEAGNIWSLWLTRR
LLDYLEANQVNLIYVAQRAEPGVERLFTVGPAPEAVPETVLQRLMAVDTGKVSVSDELWL
TEQSRLDSPLYIFTRLDDRSPTSGWLGLEVGGPDLINALQHEKAGDFMLLDAAGQLIFSS
TPDQPAAAQVLRQYHSTASFGWEGSRWLPDRLAIRKHLGYSQWQIVYSLELSSLLPSLAL
PLLVCLLLCALASLLMMRLVRRIDQRLIIPAANRIEALVESEAFSSAVIRIAPVALCVLR
RSDGEVVLENPLSRQWLGNGQERQQLCHDWIRRAFHACEQSNTDELQIADGRHLFLSFAP
IRYQREEVLICAFSDITERKQVELALQQARTLADAANEAKTLFLATMSHEIRTPLYGVLG
TLELLARTDLNSQQRGYLNAIEGSSANLLQLICDVLDVSKIEAGQLSLELSVFSPLELVQ
EVIQGYAGAAQSKGLQLFACLDPQLPDQVRGDVTRIRQILNNLLNNALKFTESGRIVLRL
RLESREGERARLQWQVVDTGKGIAAHDQQYLFEPFFQASGNANVVAGTGLGLSICKRLVH
LMNGTLRVVSEPGLGSSFTFTLPLEQVTAHEDEPWALPLQGERVYVVSPVRELAESIGGW
LRRWGARAQLGLPESVEADPGAVLVELYPGQVEPGHRLRWCGPRVTAAADEHGVYQGPGA
CWQIGLNNLHALNRAVSRAQGVEEALSQVPSEAPAKLNLNLRLLVAEDNLINQLILRDQL
EELGCSVVLAGDGIEALSLWGETAFDMILTDVNMPRMNGYELTEQLRRLGCALPIIGATA
NAMLDEAERCLSAGMDHCLVKPFTLRALYQCLQRYQRSVL