Protein Info for PFLU_RS07725 in Pseudomonas fluorescens SBW25

Annotation: Si-specific NAD(P)(+) transhydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 464 transmembrane" amino acids 180 to 199 (20 residues), see Phobius details PF07992: Pyr_redox_2" amino acids 6 to 325 (320 residues), 242.7 bits, see alignment E=3.1e-75 PF01134: GIDA" amino acids 7 to 73 (67 residues), 21.8 bits, see alignment E=5.1e-08 PF12831: FAD_oxidored" amino acids 7 to 55 (49 residues), 40.7 bits, see alignment 1.1e-13 PF00890: FAD_binding_2" amino acids 7 to 45 (39 residues), 23 bits, see alignment 2.4e-08 PF13450: NAD_binding_8" amino acids 10 to 68 (59 residues), 25.7 bits, see alignment E=6.2e-09 PF00070: Pyr_redox" amino acids 177 to 250 (74 residues), 61.3 bits, see alignment E=5.9e-20 PF02852: Pyr_redox_dim" amino acids 344 to 455 (112 residues), 88.3 bits, see alignment E=2.3e-28

Best Hits

Swiss-Prot: 100% identical to STHA_PSEFS: Soluble pyridine nucleotide transhydrogenase (sthA) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00322, NAD(P) transhydrogenase [EC: 1.6.1.1] (inferred from 100% identity to pfs:PFLU1569)

MetaCyc: 60% identical to soluble pyridine nucleotide transhydrogenase (Escherichia coli K-12 substr. MG1655)
NAD(P)H oxidase. [EC: 1.6.3.1]; 1.6.3.- [EC: 1.6.3.1]; NAD(P)(+) transhydrogenase (B-specific). [EC: 1.6.3.1, 1.6.1.1, 1.6.1.3]

Predicted SEED Role

"Soluble pyridine nucleotide transhydrogenase (EC 1.6.1.1)" in subsystem Phosphate metabolism (EC 1.6.1.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.6.1.1 or 1.6.1.3 or 1.6.3.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4W1 at UniProt or InterPro

Protein Sequence (464 amino acids)

>PFLU_RS07725 Si-specific NAD(P)(+) transhydrogenase (Pseudomonas fluorescens SBW25)
MAVYNYDVVVLGSGPAGEGAAMNAAKAGRKVAMVDSRRQVGGNCTHLGTIPSKALRHSVR
QIMQFNTNPMFRAIGEPRWFSFPDVLKSAEKVISKQVASRTGYYARNRVDLFFGTGSFAD
EQTVEVVCANGVVEKLVAKHIIIATGSRPYRPADIDFHHPRIYDSDTILSLGHTPRKLII
YGAGVIGCEYASIFSGLGVLVELVDNRDQLLSFLDSEISQALSYHFSNNNITVRHNEEYE
RVEGLDNGVILHLKSGKKIKADALLWCNGRTGNTDKLGMENIGVKVNSRGQIEVDENYRT
CVTNIYGAGDVIGWPSLASAAHDQGRSAAGSIVDNGSWRYVNDVPTGIYTIPEISSIGKN
EHELTKAKVPYEVGKAFFKSMARAQIAGEPQGMLKILFHRETLEVLGVHCFGYQASEIVH
IGQAIMSQPGEQNTLKYFVNTTFNYPTMAEAYRVAAYDGLNRLF