Protein Info for PFLU_RS07710 in Pseudomonas fluorescens SBW25
Annotation: transcription-repair coupling factor
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 57% identical to MFD_HAEIN: Transcription-repair-coupling factor (mfd) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
KEGG orthology group: K03723, transcription-repair coupling factor (superfamily II helicase) [EC: 3.6.4.-] (inferred from 55% identity to aat:D11S_0770)Predicted SEED Role
"Transcription-repair coupling factor" in subsystem DNA-replication or Transcription factors bacterial
Isozymes
Compare fitness of predicted isozymes for: 3.6.4.-
Use Curated BLAST to search for 3.6.4.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3K4V8 at UniProt or InterPro
Protein Sequence (1149 amino acids)
>PFLU_RS07710 transcription-repair coupling factor (Pseudomonas fluorescens SBW25) MPVLRLPLLPAAAGKQHWGNLPGAALSLAIAEAASAAKRFTLLLTADSQSAERLEQELSF FAPDLPVLHFPDWETLPYDLFSPHQDIISQRIASLYRLPELAHGVLVVPITTALHRLAPT KFLLGSSLVLDIGQKLDVEQMRTRLEASGYRCVDTVYEHGEFAVRGALIDLFPMGSKLPY RIDLFDDEIETLRTFDPENQRSIDKVDSIKLLPAREFPLQKDAVTRFKARFRERFDVDFR RCPIFQDLSSGITPAGIEYYLPLFFDETSTLFDYLPQDTQVFSLPGIEQAAENFWNDVRN RYEERRVDPSRPLLPPAELFLPVEDCFARLKNWPRVVASQQDVDAGSGRERFPAGTLPDL AIQAKATQPLEALSNFLGDFPGRVLFTAESAGRREVLLELLERLKLRPKTVDSWPDFVKS KERLAITIAPLDEGLLLDDPALALIAESPLFGQRVMQRRRREKRADANNDAVIKNLTELR EGAPVVHIDHGVGRYLGLQTLEIDNQAAEFLTMEYAEGAKLYVPVASLHLIARYTGSDDA LAPLHRLGSETWQKAKRKAAEQVRDVAAELLDIYARRAAREGYAFADPKADYATFSAGFA FEETPDQQTTIEAVRADMLAPKPMDRLVCGDVGFGKTEVAMRAAFIAVHGGKQVAILVPT TLLAQQHYNSFRDRFADWPVTVEVMSRFKSAKEVNAAIADLAEGKIDIVIGTHKLLSDDV KIKNLGLVIIDEEHRFGVRQKEQLKALRSEVDILTLTATPIPRTLNMAVSGMRDLSIIAT PPARRLSVRTFVMEQNKSTVKEALLRELLRGGQVYYLHNDVKTIEKCAADLAELVPEARI AIGHGQMRERDLEQVMSDFYHKRFNVLIASTIIETGIDVPSANTIIIERADKFGLAQLHQ LRGRVGRSHHQAYAYLLTPPRQQITSDAEKRLEAIANTQDLGAGFVLATNDLEIRGAGEL LGDGQSGQIQAVGFTLYMEMLERAVKAIRKGEQPNLDQPLGGGPEINLRLPALIPEDYLP DVHARLILYKRIASATDEEGLKDLQVEMIDRFGLLPEPTKNLVRLTLLKLQAEQLGIKKV DAGPQGGRIEFEAQTPVDPLVLIRLIQGQPNRYKFEGATLFKFMVPMERAEERFNTLEAL FERLIPKSA