Protein Info for PFLU_RS07700 in Pseudomonas fluorescens SBW25

Annotation: DEAD/DEAH box helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 897 PF04434: SWIM" amino acids 74 to 97 (24 residues), 23.5 bits, see alignment (E = 6.3e-09) PF04851: ResIII" amino acids 425 to 580 (156 residues), 29.6 bits, see alignment E=1.3e-10 PF00176: SNF2-rel_dom" amino acids 429 to 708 (280 residues), 249.1 bits, see alignment E=1.1e-77 PF00271: Helicase_C" amino acids 728 to 833 (106 residues), 77.8 bits, see alignment E=1.5e-25

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1563)

Predicted SEED Role

"COG0553: Superfamily II DNA/RNA helicases, SNF2 family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4V6 at UniProt or InterPro

Protein Sequence (897 amino acids)

>PFLU_RS07700 DEAD/DEAH box helicase (Pseudomonas fluorescens SBW25)
MPLTSSLTKPLAPSWANRFKEQSLERGRRYALENRVRIVECGDSTIVASCEGSGGNVYRQ
TISLRESAKGTLILVDSRCTCPVHTNCKHIAAVLLKVQETLAYPAAAQDAELLEKLQAVL
ENRVALPQVVMEDVVPVPRLWLASVEFSAFEPRNGKMQRYIQHRAALSFNYLGNYVSGQK
NADIIVRQDTQSLRIKRHPELEQPYREQLRLLGFKIATRQSKALPESAGELFEMVNDSAW
LNFTLNASPTLRAEGWELQIDEDFGFDLSTVDDWYATVDDGPERDWFDLELGIIVNGERL
SLLPILLNLMRSHTEILNPEKLARRRDEELILVNIPGLPNGGHGPLQVALPYGRLKPVLA
TLGEFYLQEAGTTTLRLAKADAIRLNPLEDLPLQWEGGEKIRNFAQRLRDIKDFTCVAPE
GLNATLRPYQLEGLSWMQSLRQLDVGGILADDMGLGKTLQTLAHILTEKIAGRLDRPCMV
VMPTSLIPNWLDEAAHFTPQLKVLALYGATRKKHFTHLQDYDLLLTTYALLPKDIEQLAA
LPLHVLILDEAQYIKNPSSKAAQAARELNARQRLCLSGTPLENHLGELWSLFHFLLPGWL
GDVKSFNRDYRVPIEKRASDVRLQHLNGRIKPFLLRRTKEQVATELPPKTEIIHWVDLNE
AQRDVYETMRLAMDKKVRDEITRKGVARSQIIILEALLKLRQVCCDLRLVNDATLPARGS
SSGKLDSLMTMLEELFEEGRRILLFSQFTSMLSLIEVELKKRGVAYALLTGQTRDRRTPV
KDFQSGKLQIFLISLKAGGVGLNLTEADTVIHYDPWWNPATENQATDRAYRIGQEKPVFV
YKMIARGTVEEKIQHLQKEKSDLAAGVLDGRTTGDWQLGNEDIEALFAPLPHKQEKR