Protein Info for PFLU_RS06925 in Pseudomonas fluorescens SBW25

Annotation: DMT family transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 299 transmembrane" amino acids 9 to 29 (21 residues), see Phobius details amino acids 36 to 56 (21 residues), see Phobius details amino acids 68 to 88 (21 residues), see Phobius details amino acids 95 to 115 (21 residues), see Phobius details amino acids 123 to 141 (19 residues), see Phobius details amino acids 147 to 166 (20 residues), see Phobius details amino acids 178 to 200 (23 residues), see Phobius details amino acids 212 to 233 (22 residues), see Phobius details amino acids 245 to 262 (18 residues), see Phobius details amino acids 268 to 284 (17 residues), see Phobius details PF00892: EamA" amino acids 7 to 131 (125 residues), 57.5 bits, see alignment E=8.4e-20 amino acids 149 to 282 (134 residues), 55 bits, see alignment E=5.1e-19

Best Hits

Swiss-Prot: 42% identical to YDEK_BACSU: Uncharacterized transporter YdeK (ydeK) from Bacillus subtilis (strain 168)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1404)

Predicted SEED Role

"Permease of the drug/metabolite transporter (DMT) superfamily" in subsystem Queuosine-Archaeosine Biosynthesis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCU8 at UniProt or InterPro

Protein Sequence (299 amino acids)

>PFLU_RS06925 DMT family transporter (Pseudomonas fluorescens SBW25)
MSSRENTGMALGLLGVIIFSLTLPFTRIVVQEIHPLLNGLGRALFAAIPAAALLLWRRER
WPTWRQVRGLCLVIAGVILGFPVLSAWAMQTLPASHGALVNGLQPLCVALYAAWLSHERP
SKAFWACAALGSALVLSYALITGAGSIQAGDLLMLGAIAVGGLGYAEGGRLAKEMGGWQV
ICWALVLSTPVLIGPVWYLAAQHQGAVSMRAWWAFGYVSLFSQFLGFFAWYAGLAMGGIA
RVSQIQLLQIFFTIAFSALFFGEHIEPITWVFACGVIVTVMLGRKTAVQPAPATKPSTV