Protein Info for PFLU_RS06820 in Pseudomonas fluorescens SBW25

Annotation: phenylacetate--CoA ligase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 95 to 303 (209 residues), 34.9 bits, E=4.1e-13

Best Hits

KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 100% identity to pfs:PFLU1382)

Predicted SEED Role

"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)

MetaCyc Pathways

• L-phenylalanine degradation IV (mammalian, via side chain) (5/9 steps found)
• penicillin G and penicillin V biosynthesis (1/4 steps found)
• phenylacetate degradation II (anaerobic) (1/4 steps found)
• superpathway of phenylethylamine degradation (6/11 steps found)
• phenylacetate degradation I (aerobic) (4/9 steps found)
• anaerobic aromatic compound degradation (Thauera aromatica) (4/27 steps found)

KEGG Metabolic Maps

• Phenylalanine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.2.1.30

Sequence Analysis Tools

See C3KBN6 at UniProt or InterPro

Protein Sequence (451 amino acids)

>PFLU_RS06820 phenylacetate--CoA ligase family protein (Pseudomonas fluorescens SBW25)
MGVESVFDEFIIDYEKIHEAYWTNTLSEYELKRWHEMQLRTVLHHVKNKSEFYARHFKDI
DVDGITLSDLGALPFTTKDDLRESMMDILSGALEDSIYYYETTGTTGPATPCPRDKKESY
ASNKQLSMAYRAVLEKHFPNEKTVVGIMGPTEVHSFGDTLGDVCHQLGVCNAKIWPHSPV
IGYPKTLQLMKDLRIGVVASAPGLLLALAKEAERLGFNPREDFHLRAFMMSGELCTPALK
NNLYSLWGCEAYNSLYGSQEAMIIAAANASDQLMPHRLNYIIEVLNPQTGESLGDSGEGE
LCVTMLIAGVKPLIRYRTGDLVCIQKNPKNPISQAQVMKVVGRVKDAMTLNGKAFTAGHI
EQALLEGVEQCLGYQIVINCVNGRDTVIAKMEMSKFYEGDRSQLTLLIRERVRERLGVDA
TIMVVDDLDDHVNLGSWFSWKEARIIDQRNR