Protein Info for PFLU_RS06310 in Pseudomonas fluorescens SBW25

Annotation: sigma E protease regulator RseP

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 transmembrane" amino acids 6 to 28 (23 residues), see Phobius details amino acids 97 to 120 (24 residues), see Phobius details amino acids 382 to 411 (30 residues), see Phobius details amino acids 423 to 444 (22 residues), see Phobius details TIGR00054: RIP metalloprotease RseP" amino acids 2 to 450 (449 residues), 403.8 bits, see alignment E=4.2e-125 PF02163: Peptidase_M50" amino acids 10 to 436 (427 residues), 254.8 bits, see alignment E=1.2e-79 PF17820: PDZ_6" amino acids 129 to 159 (31 residues), 27.2 bits, see alignment (E = 5.6e-10) amino acids 229 to 278 (50 residues), 41.3 bits, see alignment 2.2e-14 PF13180: PDZ_2" amino acids 225 to 289 (65 residues), 32.4 bits, see alignment E=1.9e-11

Best Hits

Swiss-Prot: 79% identical to Y3649_PSEAE: Putative zinc metalloprotease PA3649 (PA3649) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K11749, regulator of sigma E protease [EC: 3.4.24.-] (inferred from 100% identity to pfs:PFLU1277)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 3.4.24.-

Use Curated BLAST to search for 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K602 at UniProt or InterPro

Protein Sequence (450 amino acids)

>PFLU_RS06310 sigma E protease regulator RseP (Pseudomonas fluorescens SBW25)
MSALYMIVGTLVALGVLVTFHEFGHFWVARRCGVKVLRFSVGFGMPLLRWHDRRGTEFVI
AAIPLGGYVKMLDEREGEVPADQLDQSFNRKTVRQRIAIVAAGPIANFLLAMVFFWVLAM
LGSQQVRPVIGAVESDSIAAKAGLVAGQEIVSIDGEPTTGWGAVNLQLVRRLGESGTVNV
VVREQDSTTETPRELALDHWLKGADEPDPIKSLGIRPWRPALPPVLAELDPKGPAQAAGL
KTGDRLLALDGQSLGDWQQVVDLVRVRPETKIVLKVERDGAQIDVPVTLSVRGEAKAAGG
YLGAGVKGVDWPPSMVREVSFGPLAAIGEGAKRTWTMSVLTLESLKKMLFGELSVKNLSG
PITIAKVAGASAQSGVADFLNFLAYLSISLGVLNLLPIPVLDGGHLLFYLVEWARGRPLS
DRVQGWGIQIGISLVVGVMLLALVNDLGRL