Protein Info for PFLU_RS05955 in Pseudomonas fluorescens SBW25

Annotation: potassium transporter Kup

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 633 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details amino acids 59 to 79 (21 residues), see Phobius details amino acids 110 to 130 (21 residues), see Phobius details amino acids 150 to 168 (19 residues), see Phobius details amino acids 180 to 200 (21 residues), see Phobius details amino acids 220 to 243 (24 residues), see Phobius details amino acids 255 to 275 (21 residues), see Phobius details amino acids 295 to 320 (26 residues), see Phobius details amino acids 348 to 368 (21 residues), see Phobius details amino acids 375 to 400 (26 residues), see Phobius details amino acids 406 to 424 (19 residues), see Phobius details amino acids 430 to 451 (22 residues), see Phobius details PF02705: K_trans" amino acids 23 to 555 (533 residues), 732.2 bits, see alignment E=1.8e-224

Best Hits

Swiss-Prot: 100% identical to KUP_PSEFS: Probable potassium transport system protein kup (kup) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K03549, KUP system potassium uptake protein (inferred from 100% identity to pfs:PFLU1204)

MetaCyc: 52% identical to K+:H+ symporter Kup (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-3

Predicted SEED Role

"Kup system potassium uptake protein" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDR5 at UniProt or InterPro

Protein Sequence (633 amino acids)

>PFLU_RS05955 potassium transporter Kup (Pseudomonas fluorescens SBW25)
MGQASNQAAGAEQSNAKPIGMLVAAVGVVYGDIGTSPLYTLKEVFNGGYGVQVNHDGVLG
ILALIFWSLIWVVSIKYMLFVLRADNQGEGGIMALTALARRAAGERKKLRSFLVVCGLCG
AALFYGDSMITPAISVLSAVEGLELAFDGLEKWVVPIALVVLVALFLIQKHGTDHIGKLF
GPVMVTWFLVLGGLGVYGITLHPEVLSAMNPMWAVRFFEAHPGIGVAILGAVVLALTGAE
ALYADMGHFGRKPIARAWFILVLPALVLNYFGQGAMLLGDPEAARNPFYLLAPSWALIPL
VVLSTLATVIASQAVISGAFSLTRQAIQLGYIPRMHIQHTSSAEQGQIYIGAVNWSLMVG
VILLVLGFESSNALASAYGVAVTGTMLMTTILVSAVMLLLWKWPPVLAVPVLVCCLLVDG
LYFAANVPKIIQGGAFPVLAGIVLFVLMTTWKRGKQLLVERLDEGGLPLPIFISSIRVQP
PHRVQGTAVFLTARPDAVPHALLHNLLHNQVLHEQVVLLTVVYEDIPRVPAARRFEVDAY
GEGFFRVILHFGFTDEPDVPQALKLCHLDELDFSPMRTTYFLSRETVIASRIKGMARWRE
ALFAFMLKNANGNLRFFKLPVNRVIELGTQVEM