Protein Info for PFLU_RS05590 in Pseudomonas fluorescens SBW25

Annotation: aminotransferase class III-fold pyridoxal phosphate-dependent enzyme

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00202: Aminotran_3
  • amino acids 34 to 457 (424 residues), 243.3 bits, E=1.9e-76

Best Hits

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU1128)

Predicted SEED Role

"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)

MetaCyc Pathways

• biotin biosynthesis I (13/15 steps found)
• biotin biosynthesis from 8-amino-7-oxononanoate I (4/4 steps found)

KEGG Metabolic Maps

• Biotin metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.62

Use Curated BLAST to search for 2.6.1.62

Sequence Analysis Tools

See C3KAL6 at UniProt or InterPro

Protein Sequence (464 amino acids)

>PFLU_RS05590 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme (Pseudomonas fluorescens SBW25)
MSDIRIATAEDQILLDKEAKYCSYGDTVHYIEPPRIFSRCEGSYVWDTEDQAYLDLQMWY
SAVNFGYANPRLNNALKKQIDTLPQIASQYLHKGKIELSEMIAVDAKKKFGLDGRVHFNV
GGSQSIEDSLKVVRNATNGKSLMFAFEGGYHGRTLGASSITSSYRYRRRYGHFGERAQFI
PFPYHFRGPKGMTKEEYGSHCVQQFARLFETEYNGVWDPKVGQSEYAAFYVEPIQGTGGY
VIPPMNFYRELKQVLDQHGILMVSDEIQMGFYRTGKLWSIEHFDVQPDVIVFGKALTNGL
NPLGGIWAREELINPKIFPPGSTHSTFASNPLGTAVGLEMFKMTNEVDYGAMVMAKGKFF
LEGLQDLQKRFPIIGDVDGLGLALRCEICGPDGFTPDKATLDYMVEEGMKGDMVVDGQKL
GLILDVGGYYKNVITLAPSLEISYPEIELGLKLLEQLLVRATNR