Protein Info for PFLU_RS05355 in Pseudomonas fluorescens SBW25

Annotation: extracellular solute-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 391 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13416: SBP_bac_8" amino acids 125 to 310 (186 residues), 37.4 bits, see alignment E=1.3e-13

Best Hits

KEGG orthology group: K02055, putative spermidine/putrescine transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU1079)

Predicted SEED Role

"Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1)" in subsystem ABC transporter oligopeptide (TC 3.A.1.5.1) or Sex pheromones in Enterococcus faecalis and other Firmicutes (TC 3.A.1.5.1)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K7H5 at UniProt or InterPro

Protein Sequence (391 amino acids)

>PFLU_RS05355 extracellular solute-binding protein (Pseudomonas fluorescens SBW25)
MITLRVLGTSVTLLECLRVRAEQELGIRLVYQVHDVEQAQRIAVMQPESYDLYDQWFHNV
DFVWPARAIQPIDTRRIALWHEINDLPKRGRLSPNDRLGSGSVPSERLFVQHDGSLGSTV
TERISMLPLTHNADSFAYRPERLPEGFCHGNESWGWLLDPAWRARTALQSDAAIGALDAA
LAVQGAGLATFKDIGNMSIEEIDVLADILVRKQKEGHFAAFWSDDEEAAQLMLSPSIDIQ
SLWSPTLMRLHRAGVKYRLAVPSEGYRAWFGGVSLSRHAKGPVLDAAYAYLNWWLSGWPG
AVMARQGYYIGNPARSRDHLSSAEWDYWYAGKPAREELLGSDGLPLIDIGEMRDGGSYEQ
RMGHIAVWNSVMDEHNYLVRRWGDFMRARGG