Protein Info for PFLU_RS05310 in Pseudomonas fluorescens SBW25

Annotation: endopeptidase La

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 806 PF02190: LON_substr_bdg" amino acids 42 to 237 (196 residues), 80.4 bits, see alignment E=4.5e-26 TIGR00763: endopeptidase La" amino acids 43 to 806 (764 residues), 912.6 bits, see alignment E=1.3e-278 PF00004: AAA" amino acids 388 to 527 (140 residues), 88.4 bits, see alignment E=1.4e-28 PF07728: AAA_5" amino acids 388 to 521 (134 residues), 30.9 bits, see alignment E=6.3e-11 PF05362: Lon_C" amino acids 606 to 805 (200 residues), 253.4 bits, see alignment E=3.8e-79 PF13541: ChlI" amino acids 654 to 775 (122 residues), 32.9 bits, see alignment E=1.3e-11

Best Hits

Swiss-Prot: 62% identical to LON_MAGMM: Lon protease (lon) from Magnetococcus marinus (strain ATCC BAA-1437 / JCM 17883 / MC-1)

KEGG orthology group: K01338, ATP-dependent Lon protease [EC: 3.4.21.53] (inferred from 100% identity to pfs:PFLU1070)

Predicted SEED Role

"ATP-dependent protease La (EC 3.4.21.53) Type I" in subsystem Proteasome bacterial or Proteolysis in bacteria, ATP-dependent (EC 3.4.21.53)

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.53

Use Curated BLAST to search for 3.4.21.53

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6Z7 at UniProt or InterPro

Protein Sequence (806 amino acids)

>PFLU_RS05310 endopeptidase La (Pseudomonas fluorescens SBW25)
MSDQQDFPDYDLNDYADPENAETPSSNTGLALPGQNLPDKVYIIPIHNRPFFPAQVLPVI
VNEEPWAETLELVSKSDHHSLALFFMDTPPEDPRHFDTSSLPLYGTLVKVHHASRENGKL
QFVAQGLTRVRIKTWLKHHRPPYLVEVEYPHQPSEPTDEVKAYGMALINAIKELLPLNPL
YSEELKNYLNRFSPNDPSPLTDFAAALTSATGNELQEVLDCVPMLKRMEKVLPMLRKEVE
VARLQKELSAEVNRKIGEHQREFFLKEQLKVIQQELGLTKDDRSADVEQFEQRLEGKVLP
AQAKKRIDEELNKLSILETGSPEYAVTRNYLDWATAVPWGVYGEDKLDLKHARKVLDKHH
AGLDDIKSRILEFLAVGAYKGEVAGSIVLLVGPPGVGKTSVGKSIAESLGRPFYRFSVGG
MRDEAEIKGHRRTYIGALPGKLVQALKDVEVMNPVIMLDEIDKMGQSFQGDPASALLETL
DPEQNVEFLDHYLDLRLDLSKVLFVCTANTLDSIPGPLLDRMEVIRLSGYITEEKVAIAK
RHLWPKQLEKAGVSKNSLSISDGALRALIDGYAREAGVRQLEKQLGKLVRKAVVKLLDEP
DSVIKIGNKDLESSLGMPVFRNEQVLSGTGVITGLAWTSMGGATLPIEATRIHTLNRGFK
LTGQLGEVMKESAEIAYSYISSNLKSFGGDPKFFDEAFVHLHVPEGATPKDGPSAGVTMA
SALLSLARNQPPKKGVAMTGELTLTGHVLPIGGVREKVIAARRQKIHELILPEPNRGSFE
ELPEYLKEGMTVHFAKRFADVAKVLF