Protein Info for PFLU_RS04245 in Pseudomonas fluorescens SBW25

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 454 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 48 to 70 (23 residues), see Phobius details amino acids 79 to 97 (19 residues), see Phobius details amino acids 103 to 122 (20 residues), see Phobius details amino acids 145 to 167 (23 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details amino acids 247 to 270 (24 residues), see Phobius details amino acids 284 to 308 (25 residues), see Phobius details amino acids 320 to 338 (19 residues), see Phobius details amino acids 344 to 365 (22 residues), see Phobius details amino acids 377 to 400 (24 residues), see Phobius details amino acids 406 to 428 (23 residues), see Phobius details PF07690: MFS_1" amino acids 17 to 307 (291 residues), 174.3 bits, see alignment E=3.5e-55 TIGR00893: D-galactonate transporter" amino acids 19 to 428 (410 residues), 529.8 bits, see alignment E=2.2e-163

Best Hits

Swiss-Prot: 69% identical to GUDP_BACSU: Probable glucarate transporter (gudP) from Bacillus subtilis (strain 168)

KEGG orthology group: K03535, MFS transporter, ACS family, glucarate transporter (inferred from 100% identity to pfs:PFLU0851)

MetaCyc: 67% identical to galactarate/D-glucarate transporter GudP (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-203; TRANS-RXN0-204; TRANS-RXN0-523

Predicted SEED Role

"D-galactarate permease" in subsystem D-galactarate, D-glucarate and D-glycerate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6F2 at UniProt or InterPro

Protein Sequence (454 amino acids)

>PFLU_RS04245 MFS transporter (Pseudomonas fluorescens SBW25)
MQATKPTHVRYLILLMLFLVTTINYADRATIAIAGSSLQKDLGIDAVTLGYIFSAFGWAY
VAGQIPGGWLLDRFGSKKVYALSIFTWSLFTVLQGYVGEFGVSTAVVALFMLRFMVGLAE
APSFPGNARIVAAWFPTAERGTASAIFNSAQYFATVLFAPLMGWIVYRFGWQHVFIVMGV
IGIVFSLIWLKVIHSPRQHPMINEAELNHIAANGAMVDMDQDKGKGKKTDGPKWDYIRQL
LTNRMMLGVYLGQYCINGITYFFLTWFPVYLVQDRGMTILKAGFIASLPAICGFIGGVLG
GVISDYLLRKGHSLTFARKAPIIAGLLVSSSIIACNYVDVEWMVVGFMALAFFGKGVGAL
GWAVVSDTSPKQIAGLSGGLFNTFGNLASITTPIVIGYIISTTGSFKWALVFVGANALVA
VFSYLVIVGPIKRVVLKEPPAKGPELNNLTEAHS