Protein Info for PFLU_RS04085 in Pseudomonas fluorescens SBW25

Annotation: ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF00005: ABC_tran" amino acids 30 to 182 (153 residues), 128 bits, see alignment E=4.1e-41 PF08352: oligo_HPY" amino acids 233 to 298 (66 residues), 70.2 bits, see alignment E=1.6e-23 TIGR01727: oligopeptide/dipeptide ABC transporter, ATP-binding protein, C-terminal domain" amino acids 233 to 317 (85 residues), 90.9 bits, see alignment E=2.1e-30

Best Hits

Swiss-Prot: 61% identical to DPPF_ECOLI: Dipeptide transport ATP-binding protein DppF (dppF) from Escherichia coli (strain K12)

KEGG orthology group: K12372, dipeptide transport system ATP-binding protein (inferred from 100% identity to pfs:PFLU0819)

MetaCyc: 61% identical to dipeptide ABC transporter ATP binding subunit DppF (Escherichia coli K-12 substr. MG1655)
ABC-8-RXN [EC: 7.4.2.9]

Predicted SEED Role

"Dipeptide transport ATP-binding protein DppF (TC 3.A.1.5.2)" in subsystem ABC transporter dipeptide (TC 3.A.1.5.2) (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K5B2 at UniProt or InterPro

Protein Sequence (323 amino acids)

>PFLU_RS04085 ABC transporter ATP-binding protein (Pseudomonas fluorescens SBW25)
MAVVLTARDLTRHYEVSRGLFKGHALVRALNGVSFELEAGKTLAVVGESGCGKSTLARAL
TLIEEPSSGSLKIAGQEVAGADKAQRKQLRKDVQMVFQSPYASLNPRQKVGDQLGEPLLI
NTNLSAAERREKVQAMMKQVGLRPEHYQRYPHMFSGGQRQRIALARAMMLQPKVLVADEP
TSALDVSIQAQVLNLFMDLQQEFNTAYVFISHNLAVVQHVADDVMVMYLGRPVEVGPKED
IYARPLHPYTQALLSATPTIHPDPSKPKIKIVGELPNPLNPPPGCAFHKRCPYATQRCST
EEPQLRPLDNRQVACHYAEQFVA