Protein Info for PFLU_RS04020 in Pseudomonas fluorescens SBW25

Annotation: PTS fructose-like transporter subunit IIB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 570 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 238 to 261 (24 residues), see Phobius details amino acids 281 to 298 (18 residues), see Phobius details amino acids 310 to 333 (24 residues), see Phobius details amino acids 353 to 373 (21 residues), see Phobius details amino acids 386 to 410 (25 residues), see Phobius details amino acids 417 to 436 (20 residues), see Phobius details amino acids 438 to 456 (19 residues), see Phobius details amino acids 467 to 487 (21 residues), see Phobius details amino acids 493 to 513 (21 residues), see Phobius details amino acids 534 to 555 (22 residues), see Phobius details PF02302: PTS_IIB" amino acids 5 to 94 (90 residues), 48.6 bits, see alignment E=1e-16 amino acids 118 to 206 (89 residues), 73.3 bits, see alignment E=2.1e-24 TIGR00829: PTS system, Fru family, IIB component" amino acids 117 to 200 (84 residues), 108.6 bits, see alignment E=1.5e-35 TIGR01427: PTS system, Fru family, IIC component" amino acids 224 to 556 (333 residues), 456.2 bits, see alignment E=8.4e-141 PF02378: PTS_EIIC" amino acids 238 to 500 (263 residues), 70.7 bits, see alignment E=1.2e-23

Best Hits

Swiss-Prot: 63% identical to PTFBC_ECOLI: PTS system fructose-specific EIIB'BC component (fruA) from Escherichia coli (strain K12)

KEGG orthology group: K02769, PTS system, fructose-specific IIB component [EC: 2.7.1.69] K02770, PTS system, fructose-specific IIC component (inferred from 100% identity to pfs:PFLU0806)

MetaCyc: 63% identical to fructose-specific PTS multiphosphoryl transfer protein FruA (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69

Use Curated BLAST to search for 2.7.1.69

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4M5 at UniProt or InterPro

Protein Sequence (570 amino acids)

>PFLU_RS04020 PTS fructose-like transporter subunit IIB (Pseudomonas fluorescens SBW25)
MKLAIVTACPNGMVTSVLCARLLDAAAQRQGWSTSVEVVDVQRPERQLSQATLDDAEWVL
LVSSTPVDMQRFVGKRVFQSTPAQALADVDAVLRRGAQEAQVYVATEQAAKQAPRIVAIT
ACPTGVAHTFMAAEALQQTAKRLGYDLQVETQGSVGARTPLSPQAIAEADVVLLAADIEV
ATERFAGKKIYRCGTGIALKQSEATLNKALAEGAVESAASGAVAKKEKTGVYKHLLTGVS
FMLPMVVAGGLLIALSFVFGIHAFEQEGTLAAALKTVGDRAFMLMVPLLAGYIAYSIADR
PGIAPGMIGGLLAGTLGAGFIGGIFAGFLAGYCVKLITRAVKLPQSLEALKPILIIPLLA
SLFTGLAMIYLVGPPVARLLTGLTDFLSTMGTTNAVLLGILLGGMMCVDLGGPINKAAYA
FSVGLLAASSGAPMAATMAAGMVPPIGMGIATFLARRKFAQTEREAGKAAMILGMCFISE
GAIPFAAKDPLRVIPASIAGGALTGALSMYFGCKLAAPHGGLFVLVIPNAMNHALLYLLA
IVAGSVLTGVVYALIKRPDVVELAVAPAKA