Protein Info for PFLU_RS04010 in Pseudomonas fluorescens SBW25

Annotation: phosphoenolpyruvate--protein phosphotransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 952 transmembrane" amino acids 588 to 610 (23 residues), see Phobius details PF00359: PTS_EIIA_2" amino acids 4 to 140 (137 residues), 95.9 bits, see alignment E=5.3e-31 PF00381: PTS-HPr" amino acids 285 to 366 (82 residues), 60 bits, see alignment E=5e-20 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 402 to 950 (549 residues), 487.4 bits, see alignment E=2.7e-150 PF05524: PEP-utilisers_N" amino acids 403 to 518 (116 residues), 84.7 bits, see alignment E=1.4e-27 PF00391: PEP-utilizers" amino acids 544 to 615 (72 residues), 76.4 bits, see alignment 2.9e-25 PF02896: PEP-utilizers_C" amino acids 644 to 924 (281 residues), 357.8 bits, see alignment E=1e-110

Best Hits

KEGG orthology group: K02768, PTS system, fructose-specific IIA component [EC: 2.7.1.69] K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11183, phosphocarrier protein FPr (inferred from 100% identity to pfs:PFLU0804)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4M3 at UniProt or InterPro

Protein Sequence (952 amino acids)

>PFLU_RS04010 phosphoenolpyruvate--protein phosphotransferase (Pseudomonas fluorescens SBW25)
MLELTVEQISMGQVAVDKSAALHLLAEKLVADGLVAEGYLSGLQAREAQGSTFLGQGIAI
PHGTPETRDQVFSTGVRLLQFPEGVDWGDGQIVYLAIGIAAKSDEHLRLLQLLTRALGET
DLGQALRRAGTAEALLKLLQGAPQELALDAQMISLGVSADDFEELVWRGARLLRQADCVS
NGFAAVLQQVDALPLGDGLWWLHSEQTVKRPGLAFVTPDKPMRYLGQPLNGLFCLASLGE
AHQALLERLCALLIEGRGQELGRATSSRAVLEVLGGELPPDWPSARITLANAHGLHARPA
KILAQLAKNFDGDVRVRIVDGPVGAVSVKSLSKLLSLGARRGQVLEFVAEPTIAGDALPA
LLAAVEEGLGEDVEPLPTLSAQPEALDIEPVISAPLAGSQIQAIAAAPGIAIGPAHIQVQ
QTFDYPLRGESCAIERQRLHSALSEVRTDIQGLIERNPSKAIREIFITHQEMLDDPELTD
EVDTRLKQGESAEAAWMSVIEAAAKQQESLQDALLAERAADLRDVGRRVLAQLCGVETAQ
EPSEPYILVMDEVGPSDVARLDPARVAGILTARGGATAHSAIVARALGIPALVGAGPAVL
LLASGTPLLLDGQRGRLHVDADSATLQRATVERDTREQRLQAASAQRHEPALTRDGHAVE
VFANIGESAGVASAVEQGAEGIGLLRTELIFMAHPQAPDEATQEAEYRRVLEGLGGRPLV
VRTLDVGGDKPLPYWPIAQEENPFLGVRGIRLTLQRPQIMEAQLRALLRSADNRPLRIMF
PMVGSVDEWRAARDMTERLRLEIPVADLQLGIMIEVPSAALLAPVLAKEVDFFSVGTNDL
TQYTLAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVLI
GLGVDELSVSARSIPEVKARVREFSLSEAQGLAQKALAVGSPAEVRALVEAV