Protein Info for PFLU_RS03810 in Pseudomonas fluorescens SBW25

Annotation: isoleucine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 849 (835 residues), 898.7 bits, see alignment E=2.1e-274 PF00133: tRNA-synt_1" amino acids 27 to 646 (620 residues), 458.6 bits, see alignment E=3.6e-141 PF09334: tRNA-synt_1g" amino acids 58 to 198 (141 residues), 36.4 bits, see alignment E=5.5e-13 PF08264: Anticodon_1" amino acids 691 to 847 (157 residues), 91.7 bits, see alignment E=8.8e-30 PF06827: zf-FPG_IleRS" amino acids 905 to 932 (28 residues), 29.9 bits, see alignment (E = 7.6e-11)

Best Hits

Swiss-Prot: 100% identical to SYI_PSEFS: Isoleucine--tRNA ligase (ileS) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 92% identity to pfo:Pfl01_4852)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDX4 at UniProt or InterPro

Protein Sequence (943 amino acids)

>PFLU_RS03810 isoleucine--tRNA ligase (Pseudomonas fluorescens SBW25)
MTDYKATLNLPDTAFPMKAGLPQREPQILQRWDSIGLYGKLREIGKDRPKFVLHDGPPYA
NGTIHIGHALNKILKDMILRSKTLSGFDAPYVPGWDCHGLPIEHKVEVTYGKNLGADKTR
ELCRAYATEQIEGQKSEFIRLGVLGEWDNPYKTMNFKNEAGEIRALAEIVKGGFVFKGLK
PVNWCFDCGSALAEAEVEYEDKKSSTIDVAFPVADDAKLAEAFGLASLSKPAAIVIWTTT
PWTIPANQALNVHPEFTYALVDVGDRLLVLAEEMVEACLARYELQGSVIATTTGSALELI
NFRHPFYDRLSPVYLADYVELGAGTGIVHSAPAYGVDDFVTCKAYGMVNDDILNPVQSNG
VYAPSLEFFGGQFIFKANDSIIDKLSEVGSLLHTETIKHSYMHCWRHKTPLIYRATAQWF
IGMDKEPTSGDTLRVRSLKAIEDTQFVPAWGQARLHSMIANRPDWCISRQRNWGVPIPFF
LNKESGELHPRTVELMEEVAQRVELEGIEAWFKMDAAELLGDEAPQYDKISDTLDVWFDS
GTTHWHVLRGSHPMGHETGPRADLYLEGSDQHRGWFHSSLLTGCAIDNHAPYRELLTHGF
TVDETGRKMSKSLKNVIEPKKINDTLGADIMRLWVASTDYSGEIAVSDQILARSADAYRR
IRNTARFLLSNLTGFNPATDILPAEDMLALDRWAVDRTLLLQRELQENYGEYRFWNVYSK
IHNFCVQELGGFYLDIIKDRQYTTGANSKARRSAQTALYHISEALVRWIAPILAFTADEL
WEYLPGERNESVMLNTWYEGLTELPADFELGREYWEGVMAVKVAVNKELEVQRAAKAVGG
NLQAEVTLFAEAGLTADLAKLSNELRFVLITSTASLAPFAQAPADAVATEVPGLKLKVVK
SAFPKCARCWHCREDVGVNPEHPEICGRCVDNISGAGEVRHYA