Protein Info for PFLU_RS03690 in Pseudomonas fluorescens SBW25

Annotation: class I SAM-dependent methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 423 PF02353: CMAS" amino acids 135 to 404 (270 residues), 296.8 bits, see alignment E=3.7e-92 PF13489: Methyltransf_23" amino acids 180 to 300 (121 residues), 29 bits, see alignment E=2.1e-10 PF08241: Methyltransf_11" amino acids 198 to 294 (97 residues), 29.9 bits, see alignment E=1.7e-10 PF13649: Methyltransf_25" amino acids 198 to 291 (94 residues), 40.8 bits, see alignment E=7.3e-14 PF08242: Methyltransf_12" amino acids 198 to 292 (95 residues), 30.8 bits, see alignment E=9.8e-11

Best Hits

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 100% identity to pfs:PFLU0745)

Predicted SEED Role

"Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), plant type" (EC 2.1.1.79)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDD3 at UniProt or InterPro

Protein Sequence (423 amino acids)

>PFLU_RS03690 class I SAM-dependent methyltransferase (Pseudomonas fluorescens SBW25)
MKSPSLSVKGNRLNVNGMTTALLRKGVLRQLSQLRHGQLVVIEDGERQVFGAREAHLLGE
IQVLDSAVWGLVAANGSIGAGEAFIHGYWTSPDLTAVVRVMVSNLDVLDAMEGGLARLAR
PFTQGLHWLNRNTRKGSQKNIAAHYDLGNDLFEEFLDPTMMYSAAQFLTAEDTLEQAQLN
KLERICQKLALKPTDHLLEIGTGWGSMALYAAQHYGCKVTTTTLSKEQFAYTEQRIAALG
LQSQVTLLLKDYRDLTGQYDKLVSIEMIEAVGHRFLPTYFKQCAHLLKNDGLMLLQAITI
REQRFEQAKRSVDFIQRYIFPGGALPCVQNMLHIVSRDTDMNLLHMEDFGLHYARTLRLW
HENFRRAHGRLTELGYDEYFLRLWEFYLCYCEGGFMERTIGTAQLLLAKPSAINPPLLGR
FSA