Protein Info for PFLU_RS03475 in Pseudomonas fluorescens SBW25

Annotation: heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 478 transmembrane" amino acids 12 to 33 (22 residues), see Phobius details amino acids 163 to 186 (24 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 5 to 459 (455 residues), 449.8 bits, see alignment E=6e-139 PF00672: HAMP" amino acids 184 to 236 (53 residues), 40.9 bits, see alignment 4.1e-14 PF00512: HisKA" amino acids 242 to 304 (63 residues), 50.4 bits, see alignment E=3.7e-17 PF02518: HATPase_c" amino acids 350 to 461 (112 residues), 94.8 bits, see alignment E=9.1e-31 PF14501: HATPase_c_5" amino acids 355 to 452 (98 residues), 22.2 bits, see alignment E=2.3e-08

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to pfs:PFLU0700)

Predicted SEED Role

"Heavy metal sensor histidine kinase" in subsystem Cobalt-zinc-cadmium resistance

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KBW2 at UniProt or InterPro

Protein Sequence (478 amino acids)

>PFLU_RS03475 heavy metal sensor histidine kinase (Pseudomonas fluorescens SBW25)
MRTSSLSMRLGLTVSLMGAGLVVLLATLAYLALTHELDKLARKGLENKMEQLEHSLGQGL
DAHDISDRPHSLLDLVMGHDNIYLTIIGARPDAPVLLSVGAKPQQPLLLDVPASNTLAYL
NWVDGQGNRILSATRLMPLSSGENVRVLLSLDRSDDEALLSAYLRSTVIALPMLLILIGM
GAWWLVQRGLAPLQQFSRVAAKVSTQDLTHRLELDNLPKELGELAQGINFMLHRLDAGVQ
QLSQFSDDLAHELRAPLTNLMGKAQVTLSRERPSEEYKTSLESCTEEMERLSRMVSDMLF
LAQVSHPAARAEFAPVSLAQEAQRVMALFALSAEDKHVTLSLRGDAWVTGDRLMIQRAIS
NLLSNAIRHTPSASSVLLLVERYDQSVSLSVGNPGRGIEAHHLPHLFERFYRADSSRTRA
EGGTGLGLAIVQSIMHLHQGHADVSSQPGRFTRFRLVFPLPCDGPTDTTPATPARSAT