Protein Info for PFLU_RS03255 in Pseudomonas fluorescens SBW25

Annotation: multidrug effflux MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 43 to 60 (18 residues), see Phobius details amino acids 72 to 90 (19 residues), see Phobius details amino acids 96 to 117 (22 residues), see Phobius details amino acids 129 to 154 (26 residues), see Phobius details amino acids 160 to 179 (20 residues), see Phobius details amino acids 207 to 228 (22 residues), see Phobius details amino acids 245 to 267 (23 residues), see Phobius details amino acids 275 to 295 (21 residues), see Phobius details amino acids 301 to 321 (21 residues), see Phobius details amino acids 337 to 357 (21 residues), see Phobius details amino acids 364 to 383 (20 residues), see Phobius details TIGR00710: drug resistance transporter, Bcr/CflA subfamily" amino acids 3 to 379 (377 residues), 309.3 bits, see alignment E=2.6e-96 PF07690: MFS_1" amino acids 12 to 349 (338 residues), 134.4 bits, see alignment E=4.7e-43 PF00083: Sugar_tr" amino acids 12 to 182 (171 residues), 40.6 bits, see alignment E=1.6e-14

Best Hits

KEGG orthology group: K07552, MFS transporter, DHA1 family, bicyclomycin/chloramphenicol resistance protein (inferred from 100% identity to pfs:PFLU0661)

Predicted SEED Role

"Multidrug resistance transporter, Bcr/CflA family" in subsystem Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8V6 at UniProt or InterPro

Protein Sequence (397 amino acids)

>PFLU_RS03255 multidrug effflux MFS transporter (Pseudomonas fluorescens SBW25)
MNLRIILILGALSAFAPLAIDFYLPGFPAMATAFATDEKHIQLTLAVYFAGLAIGQLIYG
PLADRFGRRGPLLSGVTLFTLASFACAFAPSLEWLIAARFVQALGGCAGMVISRAVVSDK
CDPVGSAKVYSQLMLVTGLAPILAPLAGGLMVGVWGWQSIFLALSLFSVMAAIAVAVGLP
ETFPAHQPRQPLSGSLRRYGALLSDRVYLGYALTGGISIAGMFAYIAGSPFVFIKLYGVP
AEHYGWLFGSNAAGFILVAQVNARLLAKRGPAFLLSRTVWVYLLAALTLLGIAALRTDAL
WPLLVPLFICIASLGCILPNTSACAMSGQGARAGSASALLGCIQFGVAAGAASLVGVLHD
GTAMPMAMVISLCGVLAVTIAMSTQRLQRKRALQAQI