Protein Info for PFLU_RS03015 in Pseudomonas fluorescens SBW25

Annotation: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00355: phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase
  • amino acids 11 to 535 (525 residues), 772.5 bits, E=1.1e-236
• PF02142: MGS
  • amino acids 22 to 135 (114 residues), 82.4 bits, E=2.2e-27
• PF01808: AICARFT_IMPCHas
  • amino acids 140 to 466 (327 residues), 417.7 bits, E=3.6e-129

Best Hits

Swiss-Prot: 100% identical to PUR9_PSEFS: Bifunctional purine biosynthesis protein PurH (purH) from Pseudomonas fluorescens (strain SBW25)

KEGG orthology group: K00602, phosphoribosylaminoimidazolecarboxamide formyltransferase / IMP cyclohydrolase [EC: 2.1.2.3 3.5.4.10] (inferred from 100% identity to pfs:PFLU0612)

MetaCyc: 70% identical to bifunctional AICAR transformylase/IMP cyclohydrolase (Escherichia coli K-12 substr. MG1655)
IMP cyclohydrolase. [EC: 3.5.4.10]; Phosphoribosylaminoimidazolecarboxamide formyltransferase. [EC: 3.5.4.10, 2.1.2.3]

Predicted SEED Role

"IMP cyclohydrolase (EC 3.5.4.10) / Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3)" in subsystem De Novo Purine Biosynthesis (EC 2.1.2.3, EC 3.5.4.10)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• One carbon pool by folate
• Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.2.3 or 3.5.4.10

Sequence Analysis Tools

See C3K6E0 at UniProt or InterPro

Protein Sequence (535 amino acids)

>PFLU_RS03015 bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase PurH (Pseudomonas fluorescens SBW25)
MTDQTTRLPIRRALISVSDKTGILEFARELEALGVEILSTGGTFKLLQDNGVAAVEVADY
TGFAEMMDGRVKTLHPKIHGGILGRRGIDDAIMTEHGIKPIDLVAVNLYPFEATINKPGC
DLPTAIENIDIGGPTMVRSAAKNHKDVAIVVNASDYAQVLESLKAGGLTYAQRFDLMLKA
FEHTAAYDGMIANYMGTVNQAAETLSTEGRSQFPRTFNSQFIKAQEMRYGENPHQSAAFY
VEAKPAEVGIATATQLQGKELSYNNVADTDAALECVKSFVKPACVIVKHANPCGVAVSPD
AEGGIRQAYELAYATDTESAFGGIIAFNRELDAETAKAIVERQFVEVIIAPSVSEEARAI
VAAKANVRLLACGEWSADRAAAWDYKRVNGGLLVQSRDIGMIGSEDLKVVTKRAPTEQEI
NDLIFAWKVAKYVKSNAIVYAKNRQTIGVGAGQMSRVNSARIAAIKAEHAGLQVVGSVMA
SDAFFPFRDGLDNAAKAGVTAVIQPGGSMRDAEVIAAADEAGIAMVFTGMRHFRH