Protein Info for PFLU_RS02575 in Pseudomonas fluorescens SBW25

Annotation: GTPase HflX

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03156: GTP-binding protein HflX
  • amino acids 11 to 361 (351 residues), 512.1 bits, E=3.3e-158
• PF13167: GTP-bdg_N
  • amino acids 28 to 113 (86 residues), 110.4 bits, E=1.1e-35
• PF16360: GTP-bdg_M
  • amino acids 115 to 193 (79 residues), 101.8 bits, E=5.2e-33
• PF02421: FeoB_N
  • amino acids 200 to 324 (125 residues), 28 bits, E=3e-10
• PF01926: MMR_HSR1
  • amino acids 201 to 319 (119 residues), 72.6 bits, E=5.7e-24

Best Hits

Swiss-Prot: 60% identical to HFLX_ECOLI: GTPase HflX (hflX) from Escherichia coli (strain K12)

KEGG orthology group: K03665, GTP-binding protein HflX (inferred from 100% identity to pfs:PFLU0521)

MetaCyc: 60% identical to ribosome rescue factor HflX (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]

Predicted SEED Role

"GTP-binding protein HflX" in subsystem Hfl operon or Universal GTPases

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (12/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)

KEGG Metabolic Maps

• Purine metabolism
• Pyrimidine metabolism
• Thiamine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.15

Use Curated BLAST to search for 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Sequence Analysis Tools

See C3KDW4 at UniProt or InterPro

Protein Sequence (433 amino acids)

>PFLU_RS02575 GTPase HflX (Pseudomonas fluorescens SBW25)
MFFERHGGGERVILVHLDGQDPEAREDPQEFQELANSAGAETVAFFNVPRHRPTAKYLIG
SGKVEELRDLVHAEEADLVIFNHTLTPSQERNLERVFECRVIDRTGLILDIFAQRARTHE
GKLQVELAQLDHMSTRLVRGWTHLERQGGGIGMRGPGETQLETDRRLLRVRLRQIKGRLE
KVRSQREQSRRGRSRADIPTVSLVGYTNAGKSTLFNNVTKSDVYAADQLFATLDPTLRRL
DLDDLGPIVLADTVGFIRHLPHKLVEAFRSTLEESSNSDLLLHVIDAAEPDRMLQIEQVM
LVLGEIGAQDLPILEVYNKLDLLEGVEPQIQRDENGKPQRVWLSARDGSGLELLEQAIAE
LLGGDLFVGTLRLPQRFARLRAQFFELGAVQKEEHDEEGVSLLAVRLPRSELNRLVSREG
VVPTEFIEQHTLQ