Protein Info for PFLU_RS02380 in Pseudomonas fluorescens SBW25

Annotation: lipid A export permease/ATP-binding protein MsbA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 601 transmembrane" amino acids 23 to 50 (28 residues), see Phobius details amino acids 77 to 103 (27 residues), see Phobius details amino acids 157 to 157 (1 residues), see Phobius details amino acids 159 to 176 (18 residues), see Phobius details amino acids 181 to 199 (19 residues), see Phobius details amino acids 264 to 287 (24 residues), see Phobius details TIGR02203: lipid A export permease/ATP-binding protein MsbA" amino acids 15 to 592 (578 residues), 832.6 bits, see alignment E=8e-255 PF00664: ABC_membrane" amino acids 32 to 306 (275 residues), 169.3 bits, see alignment E=1.5e-53 PF00005: ABC_tran" amino acids 371 to 521 (151 residues), 117.4 bits, see alignment E=8.1e-38

Best Hits

Swiss-Prot: 90% identical to MSBA_PSEPF: Lipid A export ATP-binding/permease protein MsbA (msbA) from Pseudomonas fluorescens (strain Pf0-1)

KEGG orthology group: K11085, ATP-binding cassette, subfamily B, bacterial MsbA [EC: 3.6.3.-] (inferred from 100% identity to pfs:PFLU0481)

Predicted SEED Role

"Lipid A export ATP-binding/permease protein MsbA (EC 3.6.3.25)" in subsystem KDO2-Lipid A biosynthesis or Lipopolysaccharide-related cluster in Alphaproteobacteria (EC 3.6.3.25)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.25

Use Curated BLAST to search for 3.6.3.- or 3.6.3.25

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KDB7 at UniProt or InterPro

Protein Sequence (601 amino acids)

>PFLU_RS02380 lipid A export permease/ATP-binding protein MsbA (Pseudomonas fluorescens SBW25)
MTDSSPSASPSSLKIYFRLLSYVKPYAGLFALSIVGFLIFASTQPMLGYILKYFVDGLSN
PEAVLFPSVPFLRDLQLLQAVPLLIILIAAWQGLGSFLGNYLLAKVSLGLVHDLRVQLFN
NLLTLPNRYFDNHNSGHLISRITFNVTMVTGAATDAIKVVIREGMTVIFLFASLLYMNWR
LTLVMIAILPLIAVMVSTASKKFRKQSKKIQVAMGDVTHVASETIQGYRVVRSFGGEVYE
EKRFLKASQSNTDKQLRMTRTGAIYTPALQLVIYTAMAVLMFLVLYLRGDASAGDMVAYI
TLAGLLPKPIRQLSEVSSTIQKGVAGAESIFEQLDEDVEIDNGTVERDKVSGRLDVRNLN
FTYPGTERHVLKDISFTAEPGQMIALVGRSGSGKSTLASLIPRFYHHASGEILLDGVEIE
DYKLLNLRKHIAQVTQHVTLFSDTVTNNIAYGDLAGAPRADVEAAAADANAKDFIDQLPK
GFDTQVGENGVLLSGGQRQRLAIARALLKNAPLLILDEATSALDTESERHIQAALDKVMQ
GRTTLVIAHRLSTIEKADLILVMDDGRIVERGTHSELLAQNGYYARLHAMGLDAPVSADI
T