Protein Info for PFLU_RS01340 in Pseudomonas fluorescens SBW25

Annotation: RecQ family ATP-dependent DNA helicase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 647 transmembrane" amino acids 46 to 65 (20 residues), see Phobius details TIGR00614: ATP-dependent DNA helicase, RecQ family" amino acids 4 to 345 (342 residues), 373.7 bits, see alignment E=6.9e-116 PF00270: DEAD" amino acids 17 to 177 (161 residues), 77.7 bits, see alignment E=1.8e-25 PF04851: ResIII" amino acids 17 to 175 (159 residues), 25.5 bits, see alignment E=2.3e-09 PF00271: Helicase_C" amino acids 213 to 321 (109 residues), 75.4 bits, see alignment E=8.5e-25 PF16124: RecQ_Zn_bind" amino acids 514 to 555 (42 residues), 31.8 bits, see alignment 3.9e-11

Best Hits

KEGG orthology group: K03654, ATP-dependent DNA helicase RecQ [EC: 3.6.4.12] (inferred from 100% identity to pfs:PFLU0274)

Predicted SEED Role

No annotation

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K4K1 at UniProt or InterPro

Protein Sequence (647 amino acids)

>PFLU_RS01340 RecQ family ATP-dependent DNA helicase (Pseudomonas fluorescens SBW25)
MHDTLQQVFGYPQFRLGQEQTISAVLAGRSAAAIFPTGSGKSLCYQLSAVLLPHLTVVVS
PLLALMQDQLGFLQRHGISAGSIDSAQSRDEANEVMARARSGELKILMISVERLKNERFR
NFLQSVQISLLVVDEAHCISEWGHNFRPDYLKLPDYQRQFNIPQALLLTATATPKVIADM
QAKFAIAPDDVVTTGFYRPNLNLLVEPVSGSDKRRRLVEWMNERANQPSIVYVTLQKTAE
QIAEHLNRNGIQAEAYHAGLPHDKREGIQQRFMGGRSHCIVATIAFGMGIDKSDIRNVVH
FDLPKSIENYSQEIGRAGRDGQPSDCLVLANRDSLNVLENFVYGDTPEQDGIRRVLDELQ
AARSEGQWEFLLRSLSDHSNIRELPLKTLLVQLELKGVIAPRYAFYAEYRFKYLIEPEAL
LARFSGERQQFVAAIIQVCKRAKTWATVDFDALYQQHNAERGRVVKALDYFQEQGLIELE
SKQMTEVYSLLNTDFDPQALSEALYTGFKQHEVGEVARIHAMLDLFATEHCLGQRLAKYF
GDENAPQRCGHCSVCHGHVAHLPPPPSLPPLVDKNFMGLCGDFIHRHHEHTGQLPGAERL
TRFLGGISVPLFTKLKARGIPGFAALEEYPYAAVRTWAQTGLSALDQ