Protein Info for PFLU_RS01215 in Pseudomonas fluorescens SBW25

Annotation: cystine ABC transporter substrate-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00497: SBP_bac_3
  • amino acids 42 to 259 (218 residues), 242.4 bits, E=4e-76
• PF10613: Lig_chan-Glu_bd
  • amino acids 60 to 135 (76 residues), 38.2 bits, E=1.4e-13

Best Hits

Swiss-Prot: 62% identical to FLIY_ECOLI: L-cystine-binding protein FliY (fliY) from Escherichia coli (strain K12)

KEGG orthology group: K02424, cystine transport system substrate-binding protein (inferred from 100% identity to pfs:PFLU0246)

MetaCyc: 62% identical to cystine ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-290 [EC: 7.4.2.12]; 7.4.2.12 [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]; 7.4.2.- [EC: 7.4.2.12]

Predicted SEED Role

"Cystine ABC transporter, periplasmic cystine-binding protein FliY"

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.12

Sequence Analysis Tools

See C3KDU7 at UniProt or InterPro

Protein Sequence (264 amino acids)

>PFLU_RS01215 cystine ABC transporter substrate-binding protein (Pseudomonas fluorescens SBW25)
MTISVFRRTLLVGTLGLALGAGLLGQAVAGEQLGNIKKAGEIKIGLEGTYPPFSFVDESG
KLSGFEVELSEALAKELGVKVKLQATPWDGILAALESKRLDAVVNQVTISEERKKKYDFS
KPYTVSGIQALVLKKNVDTIKTADDLAGKKVGVGLGTNYEQWLKDNQPKAIIKTYNDDPT
KFQDLRIGRIDAILIDRLAALEYAKKAPDTAAAGDAFSRQEAGIALRKGEPELLAAVNQA
LDKLRADGTLKKLSEKYFNADVTQ