Protein Info for PFLU_RS01025 in Pseudomonas fluorescens SBW25
Annotation: TauD/TfdA family dioxygenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 79% identical to ATSK_PSEPU: Alpha-ketoglutarate-dependent sulfate ester dioxygenase (atsK) from Pseudomonas putida
KEGG orthology group: K03119, taurine dioxygenase [EC: 1.14.11.17] (inferred from 100% identity to pfs:PFLU0203)MetaCyc: 79% identical to type II alkyl sulfatase monomer (Pseudomonas putida)
RXN-17674 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]; 1.14.11.77 [EC: 1.14.11.77]
Predicted SEED Role
"Alpha-ketoglutarate-dependent taurine dioxygenase (EC 1.14.11.17)" in subsystem Alkanesulfonate assimilation or Taurine Utilization (EC 1.14.11.17)
MetaCyc Pathways
- taurine degradation IV (1/1 steps found)
- superpathway of taurine degradation (2/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.14.11.17
Use Curated BLAST to search for 1.14.11.17 or 1.14.11.77
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See C3KCM4 at UniProt or InterPro
Protein Sequence (301 amino acids)
>PFLU_RS01025 TauD/TfdA family dioxygenase (Pseudomonas fluorescens SBW25) MSNAALAVQPVAQALDIHPVAGRIGAEIRGIRLSGDLDAATVEAIQQALVQYKVVFFREQ THLDDQSQEAFAHLLGEPIAHPTVPVRDGTRFLMELDGTRGQRANSWHTDVTFVDAYPKA SVLRSVLAPKSGGDTVWANTSSAYNDLSVELRALADNLWAVHSNEYDYAALKPDVSAERL EAYRKVFTSTVYETEHPVVRVHPVSGEKTLLLGHFVKRLKGYSQADSTQLFNLLQSHVTR LENTVRWRWNTGDVAIWDNRATQHYAVDDYGTQERIVRRVTLKGDVPVGVHGQRSQTTKG L