Protein Info for PFLU_RS01000 in Pseudomonas fluorescens SBW25

Annotation: arylsulfatase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 536 PF00884: Sulfatase" amino acids 5 to 420 (416 residues), 217.3 bits, see alignment E=3.1e-68 PF16347: SGSH_C" amino acids 375 to 502 (128 residues), 33.5 bits, see alignment E=5e-12

Best Hits

Swiss-Prot: 80% identical to ARS_PSEAE: Arylsulfatase (atsA) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K01130, arylsulfatase [EC: 3.1.6.1] (inferred from 100% identity to pfs:PFLU0198)

MetaCyc: 80% identical to arylsulfatase (Pseudomonas aeruginosa)
Arylsulfatase. [EC: 3.1.6.1]; 3.1.6.1 [EC: 3.1.6.1]

Predicted SEED Role

"Choline-sulfatase (EC 3.1.6.6)" in subsystem Choline Transport or Choline and Betaine Uptake and Betaine Biosynthesis (EC 3.1.6.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.6.6

Use Curated BLAST to search for 3.1.6.1 or 3.1.6.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3KCL9 at UniProt or InterPro

Protein Sequence (536 amino acids)

>PFLU_RS01000 arylsulfatase (Pseudomonas fluorescens SBW25)
MPQRPNFLVILADDMGFSDLGAFGGEISTPHLDALALNGLRLTDFHTAPTCSPTRSMLLT
GTDHHIAGIGTMAEALTPELIGKPGYEGYLNDKVVALPELLREAGYQTLMSGKWHLGLTA
ELAPHARGFERSFSLLPGAANHYGFEPTYDEHTPGLLKSTPALYIEDDRFVEQLPKDFYS
SDAFGDKLLHYLKERDQARPFFAYLPFSAPHWPLQAPADIVEKYRGRYDAGPEVLRLERL
EKLKALGLIDADVEPHPLIELNTQWAALSDEQRQVSARAMEVYAAMVERMDWNIGRVVDY
LRQQGQLDNTFIVFMSDNGAEGALLEAFPKFGPQLLTYLNQHYDNSLDNIGRANSYVWYG
PSWAQVATAPSRLFKAFTTEGGIRVPALVHYPQLSLKGRISHGFGTVMDITPTILDLAGV
RHPGKQWRGKPVAPLRGKSWLGFLSGETAQVHDDDTVTGWELFGRRAIRQGQWKAVYIPG
PVGPATWQLYDLGQDPGEIHDLALSHPDKLARLIGHWQQYVDETGVVLSASPFQPD