Protein Info for PFLU_RS00715 in Pseudomonas fluorescens SBW25

Annotation: efflux RND transporter periplasmic adaptor subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details PF00529: CusB_dom_1" amino acids 20 to 268 (249 residues), 27.6 bits, see alignment E=4.7e-10 PF16576: HlyD_D23" amino acids 46 to 237 (192 residues), 42.7 bits, see alignment E=8.4e-15 TIGR01730: efflux transporter, RND family, MFP subunit" amino acids 46 to 229 (184 residues), 116.6 bits, see alignment E=5.6e-38 PF13533: Biotin_lipoyl_2" amino acids 48 to 95 (48 residues), 55.4 bits, see alignment 8e-19 PF13437: HlyD_3" amino acids 155 to 237 (83 residues), 51.1 bits, see alignment E=4e-17

Best Hits

Swiss-Prot: 42% identical to YDHJ_ECOLI: Uncharacterized protein YdhJ (ydhJ) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to pfs:PFLU0139)

MetaCyc: 40% identical to aromatic carboxylic acid efflux pump membrane fusion protein (Escherichia coli K-12 substr. MG1655)
TRANS-RXN0-233

Predicted SEED Role

"HlyD family secretion protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K8T4 at UniProt or InterPro

Protein Sequence (296 amino acids)

>PFLU_RS00715 efflux RND transporter periplasmic adaptor subunit (Pseudomonas fluorescens SBW25)
MKKPFLTIGRVVLTLLIVTFAVVVVWRMVMYYMFAPWTRDGHIRADIVQIAPDVSGLIQR
VDVRDNQLVTKGQVLFAVDQDRFKLALRQAQAAVADRQETLAQAQREYKRNRGLGNLVPS
EQLEESQSRVARAQSALAEAQVTVDSAQLNLDRSVIRSPVDGYVNDRAPRAQEFVTAGRP
VLSVVDSNSFHIDGYFEETKLDGIHVGMSVDIRVIGDNARLRGHVQSIVAGIEDRDRSSG
SNLLPNVNPAFSWVRLAQRIPVRIAFDDVPADFRMIAGRTATVSIIGDKVDNGAKP