Protein Info for PFLU_RS00395 in Pseudomonas fluorescens SBW25

Annotation: DNA polymerase I

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 925 PF02739: 5_3_exonuc_N" amino acids 6 to 167 (162 residues), 181.8 bits, see alignment E=1.7e-57 TIGR00593: DNA polymerase I" amino acids 6 to 925 (920 residues), 945.8 bits, see alignment E=1.6e-288 PF01367: 5_3_exonuc" amino acids 168 to 270 (103 residues), 101.9 bits, see alignment E=4.8e-33 PF01612: DNA_pol_A_exo1" amino acids 318 to 511 (194 residues), 122.1 bits, see alignment E=4.4e-39 PF00476: DNA_pol_A" amino acids 548 to 922 (375 residues), 545.9 bits, see alignment E=1e-167

Best Hits

KEGG orthology group: K02335, DNA polymerase I [EC: 2.7.7.7] (inferred from 100% identity to pfs:PFLU0079)

Predicted SEED Role

"DNA polymerase I (EC 2.7.7.7)" in subsystem DNA-replication or DNA Repair Base Excision (EC 2.7.7.7)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.7.7

Use Curated BLAST to search for 2.7.7.7

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See C3K6S9 at UniProt or InterPro

Protein Sequence (925 amino acids)

>PFLU_RS00395 DNA polymerase I (Pseudomonas fluorescens SBW25)
MSQAPLVLVDGSSYLYRAFHALPPLTTSKGLPTGAVKGVLNMLKSLRKQYPDSPFAVVFD
AKGGTFRDDMYAEYKANRPSMPDDMRLQIEPLHQSVIALGFPLLCVEGVEADDVIGTLAR
SSAAADRPVVISTGDKDMAQLVDGHITLVNTMTGSAMDIEGVKEKFGVAPEQIIDYLALM
GDSSDNIPGVPGIGPKTASGLLVGVNGGLKELYEQLDIVPTLPIRGAKTLPAKLEEHKEM
AFLSYQLATIKIDVPLDVGLDDLHLIEPDREKLLELYTLLEFKSWFDEIQRDAKRVELKA
APVVEDVAEVAAPAEATYTTILDQATFDIWLKKLNDAKLFAFDTETTGIDAQQAQLVGVS
FAVQPHEAAYIPLTHAYIGAPEQLDRDTVLLALKPLLEDPTKLKVGQHAKFDMNILANCA
IGGDPAHGITVRGIAFDTMLESYVLNATATRHDMDSLAKKYLDYDTVAFQDIAGKGAKQL
TFDQIALEQAGPYAAEDADVTLRLHQALFAQLSAIPSLASVLTDIEIPLVPVLARIERQG
ALVDKDLLGIQSIELGNKMVELERQAFEIAGEEFNLGSPKQLGAILYEKLGLPVLKKTGK
GQASTAEEVLAKLAEDDYPLPKVLMQYRSMSKLKSTYTDRLPEQINPRTGRIHTSYHQAV
AATGRLSSSDPNLQNIPVRTAEGRRIRQAFIAPKGYKMLAADYSQIELRIMAHLSKDEGL
MNAFRHNLDVHTATAAEVFKVELGDVTSDQRRSAKAINFGLIYGMGAQKLGKDIGVDTKT
AKAYIDVYFARYPGVREYMERTRAQASDQGYVETFFGRRLYLPDIHSNKPQERAAAERTA
INAPMQGTAADIIKKAMVLVDNWLTDSGLDAKVILQVHDELVLEVREDLVAEVSEKIREY
MSAAAQLDVPLLVEVGVGDNWDQAH